CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > Neurophoton. > Volume 5 > Issue 4 > Article
Open Access
3 November 2018 Fully automated dual-resolution serial optical coherence tomography aimed at diffusion MRI validation in whole mouse brains
Joël Lefebvre, Patrick Delafontaine-Martel, Philippe Pouliot, Hélène Girouard, Maxime Descoteaux, Frédéric Lesage
Author Affiliations +
Funded by: Fonds de Recherche du Québec—Nature et Technologies, Canadian Institutes of Health Research, Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada (NSERC Canadian Network for Research and Innovation in Machining Technology)
Neurophotonics, Vol. 5, Issue 4, 045004 (November 2018). https://doi.org/10.1117/1.NPh.5.4.045004
Abstract
An automated dual-resolution serial optical coherence tomography (2R-SOCT) scanner is developed. The serial histology system combines a low-resolution (25  μm  /  voxel) 3  ×   OCT with a high-resolution (1.5  μm  /  voxel) 40  ×   OCT to acquire whole mouse brains at low resolution and to target specific regions of interest (ROIs) at high resolution. The 40  ×   ROIs positions are selected either manually by the microscope operator or using an automated ROI positioning selection algorithm. Additionally, a multimodal and multiresolution registration pipeline is developed in order to align the 2R-SOCT data onto diffusion MRI (dMRI) data acquired in the same ex vivo mouse brains prior to automated histology. Using this imaging system, 3 whole mouse brains are imaged, and 250 high-resolution 40  ×   three-dimensional ROIs are acquired. The capability of this system to perform multimodal imaging studies is demonstrated by labeling the ROIs using a mouse brain atlas and by categorizing the ROIs based on their associated dMRI measures. This reveals a good correspondence of the tissue microstructure imaged by the high-resolution OCT with various dMRI measures such as fractional anisotropy, number of fiber orientations, apparent fiber density, orientation dispersion, and intracellular volume fraction.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Citation Download Citation
Joël Lefebvre, Patrick Delafontaine-Martel, Philippe Pouliot, Hélène Girouard, Maxime Descoteaux, and Frédéric Lesage "Fully automated dual-resolution serial optical coherence tomography aimed at diffusion MRI validation in whole mouse brains," Neurophotonics 5(4), 045004 (3 November 2018). https://doi.org/10.1117/1.NPh.5.4.045004
Received: 23 July 2018; Accepted: 12 October 2018; Published: 3 November 2018
JOURNAL ARTICLE
 16 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 8 scholarly publications.
Advertisement
Advertisement
KEYWORDS
Brain
Optical coherence tomography
Tissues
Neuroimaging
Diffusion magnetic resonance imaging
Image registration
Image resolution
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top