Simulation study of second-harmonic microscopic imaging signals through tissue-like turbid media

Xiao-Yuan Deng; Xian-Ju Wang; Hanping Liu; Zhengfei Zhuang; Zhou-Yi Guo

doi:10.1117/1.2191055

1 March 2006 Simulation study of second-harmonic microscopic imaging signals through tissue-like turbid media

Xiao-Yuan Deng, Xian-Ju Wang, Hanping Liu, Zhengfei Zhuang, Zhou-Yi Guo

Author Affiliations +

Journal of Biomedical Optics, Vol. 11, Issue 2, 024013 (March 2006). https://doi.org/10.1117/1.2191055

Abstract

We establish, for the first time, a simulation model for dealing with the second-harmonic signals under a microscope through a tissue-like turbid medium, based on the Monte Carlo method. With this model, the angle-resolved distribution and the signal level η of second-harmonic light through a slab of the turbid medium are demonstrated and the effects of the thickness (d) of the turbid medium, the numerical aperture (NA) of the objective as well as the size (ρ) of the scatterers forming the turbid medium are explored. Simulation results reveal that the use of a small objective NA results in a narrow angle distribution but strong second-harmonic signals. A turbid medium consisting of large scattering particles has a strong influence on the angle distribution and the signal level η, which results in a low penetration limit for second-harmonic signals made up of ballistic photons. It is approximately 30 µm in our situation.

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Xiao-Yuan Deng, Xian-Ju Wang, Hanping Liu, Zhengfei Zhuang, and Zhou-Yi Guo "Simulation study of second-harmonic microscopic imaging signals through tissue-like turbid media," Journal of Biomedical Optics 11(2), 024013 (1 March 2006). https://doi.org/10.1117/1.2191055

Published: 1 March 2006

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KEYWORDS

Scattering

Monte Carlo methods

Second-harmonic generation

Particles

Photons

Light scattering

Luminescence

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