Determination of optical properties of human blood in the spectral range 250  to  1100  nm using Monte Carlo simulations with hematocrit-dependent effective scattering phase functions

Moritz Friebel; Andre Roggan; Gerhard J. Müller; Martina C. Meinke

doi:10.1117/1.2203659

1 May 2006 Determination of optical properties of human blood in the spectral range 250 to 1100 nm using Monte Carlo simulations with hematocrit-dependent effective scattering phase functions

Moritz Friebel, Andre Roggan, Gerhard J. Müller, Martina C. Meinke

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Journal of Biomedical Optics, Vol. 11, Issue 3, 034021 (May 2006). https://doi.org/10.1117/1.2203659

Abstract

The absorption coefficient µ_a, scattering coefficient µ_s, and anisotropy factor g of diluted and undiluted human blood (hematocrit 0.84 and 42.1%) are determined under flow conditions in the wavelength range 250 to 1100 nm, covering the absorption bands of hemoglobin. These values are obtained by high precision integrating sphere measurements in combination with an optimized inverse Monte Carlo simulation (IMCS). With a new algorithm, appropriate effective phase functions could be evaluated for both blood concentrations using the IMCS. The best results are obtained using the Reynolds-McCormick phase function with the variation factor α=1.2 for hematocrit 0.84%, and α=1.7 for hematocrit 42.1%. The obtained data are compared with the parameters given by the Mie theory. The use of IMCS in combination with selected appropriate effective phase functions make it possible to take into account the nonspherical shape of erythrocytes, the phenomenon of coupled absorption and scattering, and multiple scattering and interference phenomena. It is therefore possible for the first time to obtain reasonable results for the optical behavior of human blood, even at high hematocrit and in high hemoglobin absorption areas. Moreover, the limitations of the Mie theory describing the optical properties of blood can be shown.

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Moritz Friebel, Andre Roggan, Gerhard J. Müller, and Martina C. Meinke "Determination of optical properties of human blood in the spectral range 250 to 1100 nm using Monte Carlo simulations with hematocrit-dependent effective scattering phase functions," Journal of Biomedical Optics 11(3), 034021 (1 May 2006). https://doi.org/10.1117/1.2203659

Published: 1 May 2006

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