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1 May 2009 Three-dimensional surface profile intensity correction for spatially modulated imaging
Sylvain Gioux, Amaan Mazhar, David J. Cuccia, Anthony Joseph Durkin, Bruce Jason Tromberg, John V. Frangioni M.D.
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Journal of Biomedical Optics, Vol. 14, Issue 3, 034045 (May 2009). https://doi.org/10.1117/1.3156840
Abstract
We describe a noncontact profile correction technique for quantitative, wide-field optical measurement of tissue absorption (μa) and reduced scattering (μs′) coefficients, based on geometric correction of the sample's Lambertian (diffuse) reflectance intensity. Because the projection of structured light onto an object is the basis for both phase-shifting profilometry and modulated imaging, we were able to develop a single instrument capable of performing both techniques. In so doing, the surface of the three-dimensional object could be acquired and used to extract the object's optical properties. The optical properties of flat polydimethylsiloxane (silicone) phantoms with homogenous tissue-like optical properties were extracted, with and without profilometry correction, after vertical translation and tilting of the phantoms at various angles. Objects having a complex shape, including a hemispheric silicone phantom and human fingers, were acquired and similarly processed, with vascular constriction of a finger being readily detectable through changes in its optical properties. Using profilometry correction, the accuracy of extracted absorption and reduced scattering coefficients improved from two- to ten-fold for surfaces having height variations as much as 3 cm and tilt angles as high as 40 deg. These data lay the foundation for employing structured light for quantitative imaging during surgery.
©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Sylvain Gioux, Amaan Mazhar, David J. Cuccia, Anthony Joseph Durkin, Bruce Jason Tromberg, and John V. Frangioni M.D. "Three-dimensional surface profile intensity correction for spatially modulated imaging," Journal of Biomedical Optics 14(3), 034045 (1 May 2009). https://doi.org/10.1117/1.3156840
Published: 1 May 2009
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Cited by 137 scholarly publications and 5 patents.
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KEYWORDS
Optical properties
Absorption
Scattering
Calibration
Spatial frequencies
Modulation
Optical testing
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