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1 May 2009 Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy
Benjamin Rappaz, Elena Cano, Tristan Colomb, Jonas Kuhn, Christian D. Depeursinge, Viesturs Simanis, Pierre J. Magistretti, Pierre P. Marquet
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Journal of Biomedical Optics, Vol. 14, Issue 3, 034049 (May 2009). https://doi.org/10.1117/1.3147385
Abstract
Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, including dry mass production, density, and spatial distribution. We have applied DHM to study the dry mass production rate and the dry mass surface density in wild-type and mutant fission yeast cells. Our study demonstrates the applicability of DHM as a tool for label-free quantitative analysis of the cell cycle and opens the possibility for its use in high-throughput screening.
©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Benjamin Rappaz, Elena Cano, Tristan Colomb, Jonas Kuhn, Christian D. Depeursinge, Viesturs Simanis, Pierre J. Magistretti, and Pierre P. Marquet "Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy," Journal of Biomedical Optics 14(3), 034049 (1 May 2009). https://doi.org/10.1117/1.3147385
Published: 1 May 2009
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Cited by 187 scholarly publications and 4 patents.
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KEYWORDS
Yeast
Digital holography
Microscopy
Holography
Phase shifts
Brain
Image processing
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