Deep in vivo two-photon imaging of blood vessels with a new dye encapsulated in pluronic nanomicelles

Mathieu Maurin; Olivier Stephan; Jean-Claude A. Vial; Seth R. Marder; Boudewijn P. J. van der Sanden

doi:10.1117/1.3548879

1 March 2011 Deep in vivo two-photon imaging of blood vessels with a new dye encapsulated in pluronic nanomicelles

Mathieu Maurin, Olivier Stephan, Jean-Claude A. Vial, Seth R. Marder, Boudewijn P. J. van der Sanden

Author Affiliations +

Journal of Biomedical Optics, Vol. 16, Issue 3, 036001 (March 2011). https://doi.org/10.1117/1.3548879

Abstract

Our purpose is to test if Pluronic® fluorescent nanomicelles can be used for in vivo two-photon imaging of both the normal and the tumor vasculature. The nanomicelles were obtained after encapsulating a hydrophobic two-photon dye: di-stryl benzene derivative, in Pluronic block copolymers. Their performance with respect to imaging depth, blood plasma staining, and diffusion across the tumor vascular endothelium is compared to a classic blood pool dye Rhodamin B dextran (70 kDa) using two-photon microscopy. Pluronic nanomicelles show, like Rhodamin B dextran, a homogeneous blood plasma staining for at least 1 h after intravenous injection. Their two-photon imaging depth is similar in normal mouse brain, using 10 times less injected mass. In contrast with Rhodamin B dextran, no extravasation is observed in leaky tumor vessels due to their large size: 20-100 nm. In conclusion, Pluronic nanomicelles can be used as a blood pool dye, even in leaky tumor vessels. The use of Pluronic block copolymers is a valuable approach for encapsulating two-photon fluorescent dyes that are hydrophobic and not suitable for intravenous injection.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Mathieu Maurin, Olivier Stephan, Jean-Claude A. Vial, Seth R. Marder, and Boudewijn P. J. van der Sanden "Deep in vivo two-photon imaging of blood vessels with a new dye encapsulated in pluronic nanomicelles," Journal of Biomedical Optics 16(3), 036001 (1 March 2011). https://doi.org/10.1117/1.3548879

Published: 1 March 2011

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KEYWORDS

Blood

Tumors

Two photon imaging

In vivo imaging

Blood vessels

Luminescence

Plasma

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