Rhodamine 800 as a near-infrared fluorescent deposition flow tracer in rodent hearts

Garret Munch; Scott McKay; Eugene Gussakovsky; Bozena Kuzio; Valery V. Kupriyanov; Olga Jilkina

doi:10.1117/1.3583581

1 June 2011 Rhodamine 800 as a near-infrared fluorescent deposition flow tracer in rodent hearts

Garret Munch, Scott McKay, Eugene Gussakovsky, Bozena Kuzio, Valery V. Kupriyanov, Olga Jilkina

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Journal of Biomedical Optics, Vol. 16, Issue 6, 065001 (June 2011). https://doi.org/10.1117/1.3583581

Abstract

We investigated the use of a near-infrared (NIR) fluorescent dye, Rhodamine 800 (Rhod800, λ_exc = 693 nm, λ_em > 720 nm) as a flow-dependent molecular tracer for NIR spectroscopy and high-resolution cardiac imaging. Rhod800 accumulates in isolated mitochondria in proportion to the mitochondrial membrane potential (Δψ). However, in the intact myocardium, Rhod800 binding is Δψ-independent. Rat hearts were perfused in a Langendorff mode with Krebs-Henseleit buffer containing 45-nM Rhod800 at normal (100%), increased (150%), or reduced (50%) baseline coronary flow (CF) per gram, for 30 to 60 min. In a different group of hearts, the left anterior descending artery (LAD) was occluded prior to Rhod800 infusion to create a flow deficit area. Rhod800 deposition was analyzed by: 1. absorbance spectroscopy kinetics in the Rhod800-perfused hearts, 2. Rhod800 absorbance and fluorescence imaging in the short-axis heart slices, and 3. dynamic epicardial/subepicardial fluorescence imaging of Rhod800 in KCl-arrested hearts, with a spatial resolution of ~200µm. Rhod800 deposition was proportional to the perfusate volume (CF and perfusion time) and there was no Rhod800 loss during the washout period. In the LAD-ligated hearts, Rhod800 fluorescence was missing from the no-flow, LAD-dependent endocardial and epicardial/subepicardial area. We concluded that Rhod800 can be used as a deposition flow tracer for dynamic cardiac imaging.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Garret Munch, Scott McKay, Eugene Gussakovsky, Bozena Kuzio, Valery V. Kupriyanov, and Olga Jilkina "Rhodamine 800 as a near-infrared fluorescent deposition flow tracer in rodent hearts," Journal of Biomedical Optics 16(6), 065001 (1 June 2011). https://doi.org/10.1117/1.3583581

Published: 1 June 2011

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