Wavelength-resolved measurements of fluorescence lifetime of indocyanine green

Anna Gerega; Norbert Zolek; Tomasz Soltysinski; Daniel Milej; Piotr Sawosz; Beata Toczylowska; Adam Liebert

doi:10.1117/1.3593386

1 June 2011 Wavelength-resolved measurements of fluorescence lifetime of indocyanine green

Anna Gerega, Norbert Zolek, Tomasz Soltysinski, Daniel Milej, Piotr Sawosz, Beata Toczylowska, Adam Liebert

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Journal of Biomedical Optics, Vol. 16, Issue 6, 067010 (June 2011). https://doi.org/10.1117/1.3593386

Abstract

We study fluorescence lifetime of indocyanine green (ICG) using femtosecond laser and sensitive detection based on time-correlated single-photon counting. A time-resolved multichannel spectral system is constructed and applied for determination of the fluorescence lifetime of the ICG in different solvents. Emission properties of ICG in water, milk, and 1% intralipid solution are investigated. Fluorescence of the fluorophore of different concentrations (in a range of 1.7-160 μM) dissolved in different solutions is excited by femtosecond pulses generated with the use of Ti:Sa laser tuned within the range of 740-790 nm. It is observed that fluorescence lifetime of ICG in water is 0.166 ± 0.02 ns and does not depend on excitation and emission wavelengths. We also show that for the diffusely scattering solvents (milk and intralipid), the lifetime may depend on the dye concentration (especially for large concentrations of ICG). This effect should be taken into account when analyzing changes in the mean time of arrival of fluorescence photons excited in ICG dissolved in such optically turbid media.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Anna Gerega, Norbert Zolek, Tomasz Soltysinski, Daniel Milej, Piotr Sawosz, Beata Toczylowska, and Adam Liebert "Wavelength-resolved measurements of fluorescence lifetime of indocyanine green," Journal of Biomedical Optics 16(6), 067010 (1 June 2011). https://doi.org/10.1117/1.3593386

Published: 1 June 2011

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