CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 17 > Issue 11 > Article
Open Access
1 November 2012 Label-free assessment of adipose-derived stem cell differentiation using coherent anti-Stokes Raman scattering and multiphoton microscopy
Rabah Mouras, Pierre O. Bagnaninchi, Andrew R. Downes, Alistair P. D Elfick
Author Affiliations +
Journal of Biomedical Optics, Vol. 17, Issue 11, 116011 (November 2012). https://doi.org/10.1117/1.JBO.17.11.116011
Abstract
Adult stem cells (SCs) hold great potential as likely candidates for disease therapy but also as sources of differentiated human cells in vitro models of disease. In both cases, the label-free assessment of SC differentiation state is highly desirable, either as a quality-control technology ensuring cells to be used clinically are of the desired lineage or to facilitate in vitro time-course studies of cell differentiation. We investigate the potential of nonlinear optical microscopy as a minimally invasive technology to monitor the differentiation of adipose-derived stem cells (ADSCs) into adipocytes and osteoblasts. The induction of ADSCs toward these two different cell lineages was monitored simultaneously using coherent anti-Stokes Raman scattering, two photon excitation fluorescence (TPEF), and second harmonic generation at different time points. Changes in the cell's morphology, together with the appearance of biochemical markers of cell maturity were observed, such as lipid droplet accumulation for adipo-induced cells and the formation of extra-cellular matrix for osteo-induced cells. In addition, TPEF of flavoproteins was identified as a proxy for changes in cell metabolism that occurred throughout ADSC differentiation toward both osteoblasts and adipocytes. These results indicate that multimodal microscopy has significant potential as an enabling technology for the label-free investigation of SC differentiation.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
Citation Download Citation
Rabah Mouras, Pierre O. Bagnaninchi, Andrew R. Downes, and Alistair P. D Elfick "Label-free assessment of adipose-derived stem cell differentiation using coherent anti-Stokes Raman scattering and multiphoton microscopy," Journal of Biomedical Optics 17(11), 116011 (1 November 2012). https://doi.org/10.1117/1.JBO.17.11.116011
Published: 1 November 2012
JOURNAL ARTICLE
 8 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 27 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Collagen
Second-harmonic generation
Stem cells
Microscopy
Multiphoton microscopy
CARS tomography
Luminescence
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top