Development of a multiplexing fingerprint and high wavenumber Raman spectroscopy technique for real-time in vivo tissue Raman measurements at endoscopy

Mads Sylvest Bergholt; Wei Zheng; Zhiwei Huang

doi:10.1117/1.JBO.18.3.030502

28 February 2013 Development of a multiplexing fingerprint and high wavenumber Raman spectroscopy technique for real-time in vivo tissue Raman measurements at endoscopy

Mads Sylvest Bergholt, Wei Zheng, Zhiwei Huang

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Journal of Biomedical Optics, Vol. 18, Issue 3, 030502 (February 2013). https://doi.org/10.1117/1.JBO.18.3.030502

Abstract

We report on the development of a novel multiplexing Raman spectroscopy technique using a single laser light together with a volume phase holographic (VPH) grating that simultaneously acquires both fingerprint (FP) and high wavenumber (HW) tissue Raman spectra at endoscopy. We utilize a customized VPH dual-transmission grating, which disperses the incident Raman scattered light vertically onto two separate segments (i.e., −150 to 1950 cm^−[sup]1 ; 1750 to 3600 cm ^−[sup]1 ) of a charge-coupled device camera. We demonstrate that the multiplexing Raman technique can acquire high quality in vivo tissue Raman spectra ranging from 800 to 3600 cm ⁻¹ within 1.0 s with a spectral resolution of 3 to 6 cm⁻¹ during clinical endoscopy. The rapid multiplexing Raman spectroscopy technique covering both FP and HW ranges developed in this work has potential for improving in vivo tissue diagnosis and characterization at endoscopy.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Mads Sylvest Bergholt, Wei Zheng, and Zhiwei Huang "Development of a multiplexing fingerprint and high wavenumber Raman spectroscopy technique for real-time in vivo tissue Raman measurements at endoscopy," Journal of Biomedical Optics 18(3), 030502 (28 February 2013). https://doi.org/10.1117/1.JBO.18.3.030502

Published: 28 February 2013

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