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24 May 2013 Polarization control of Raman spectroscopy optimizes the assessment of bone tissue
Alexander J. Makowski, Chetan A. Patil, Anita Mahadevan-Jansen, Jeffry S. Nyman
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Funded by: NSF
Journal of Biomedical Optics, Vol. 18, Issue 5, 055005 (May 2013). https://doi.org/10.1117/1.JBO.18.5.055005
Abstract
There is potential for Raman spectroscopy (RS) to complement tools for bone diagnosis due to its ability to assess compositional and organizational characteristics of both collagen and mineral. To aid this potential, the present study assessed specificity of RS peaks to the composition of bone, a birefringent material, for different degrees of instrument polarization. Specifically, relative changes in peaks were quantified as the incident light rotated relative to the orientation of osteonal and interstitial tissue, acquired from cadaveric femurs. In a highly polarized instrument (10 6 ∶1 extinction ratio), the most prominent mineral peak (ν1 Phosphate at 961  cm −1 ) displayed phase similarity with the Proline peak at 856  cm −1[/sup] . This sensitivity to relative orientation between bone and light observed in the highly polarized regime persisted for certain sensitive peaks (e.g., Amide I at 1666  cm −1 ) in unaltered instrumentation (200∶1 extinction ratio). Though Proline intensity changed with bone rotation, the phase of Proline matched that of ν1 Phosphate. Moreover, when mapping ν1 Phosphate/Proline across osteonal-interstitial borders, the mineralization difference between the tissue types was evident whether using a 20x or 50x objectives. Thus, the polarization bias inherent in commercial RS systems does not preclude the assessment of bone composition when using phase-matched peaks.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Alexander J. Makowski, Chetan A. Patil, Anita Mahadevan-Jansen, and Jeffry S. Nyman "Polarization control of Raman spectroscopy optimizes the assessment of bone tissue," Journal of Biomedical Optics 18(5), 055005 (24 May 2013). https://doi.org/10.1117/1.JBO.18.5.055005
Published: 24 May 2013
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Cited by 49 scholarly publications.
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KEYWORDS
Polarization
Bone
Remote sensing
Raman spectroscopy
Collagen
Minerals
Tissues
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