CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 18 > Issue 9 > Article
Open Access
3 September 2013 Needle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objective
Matthew R. Kyrish, Jessica L. Dobbs, Shalini Jain, Xiao Wang, Dihua Yu, Rebecca R. Richards-Kortum, Tomasz S. Tkaczyk
Author Affiliations +
Journal of Biomedical Optics, Vol. 18, Issue 9, 096003 (September 2013). https://doi.org/10.1117/1.JBO.18.9.096003
Abstract
In order to diagnose cancer, a sample must be removed, prepared, and examined under a microscope, which is expensive, invasive, and time consuming. Fiber optic fluorescence endomicroscopy, where an image guide is used to obtain high-resolution images of tissue in vivo, has shown promise as an alternative to conventional biopsies. However, the resolution of standard endomicroscopy is limited by the fiber bundle sampling frequency and out-of-focus light. A system is presented which incorporates a plastic, achromatic objective to increase the sampling and which provides optical sectioning via structured illumination to reject background light. An image is relayed from the sample by a fiber bundle with the custom 2.1-mm outer diameter objective lens integrated to the distal tip. The objective is corrected for the excitation and the emission wavelengths of proflavine (452 and 515 nm). It magnifies the object onto the fiber bundle to improve the system’s lateral resolution by increasing the sampling. The plastic lenses were fabricated via single-point diamond turning and assembled using a zero alignment technique. Ex vivo images of normal and neoplastic murine mammary tissues stained with proflavine are captured. The system achieves higher contrast and resolves smaller features than standard fluorescence endomicroscopy.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2013/$25.00 © 2013 SPIE
Citation Download Citation
Matthew R. Kyrish, Jessica L. Dobbs, Shalini Jain, Xiao Wang, Dihua Yu, Rebecca R. Richards-Kortum, and Tomasz S. Tkaczyk "Needle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objective," Journal of Biomedical Optics 18(9), 096003 (3 September 2013). https://doi.org/10.1117/1.JBO.18.9.096003
Published: 3 September 2013
JOURNAL ARTICLE
 8 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 28 scholarly publications and 1 patent.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Objectives
Tissues
Luminescence
Endomicroscopy
Microscopes
Tissue optics
Image resolution
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top