Fast spectral coherent anti-Stokes Raman scattering microscopy with high-speed tunable picosecond laser

Harsono Cahyadi; Junichi Iwatsuka; Takeo Minamikawa; Hirohiko Niioka; Tsutomu Araki; Mamoru Hashimoto

doi:10.1117/1.JBO.18.9.096009

6 September 2013 Fast spectral coherent anti-Stokes Raman scattering microscopy with high-speed tunable picosecond laser

Harsono Cahyadi, Junichi Iwatsuka, Takeo Minamikawa, Hirohiko Niioka, Tsutomu Araki, Mamoru Hashimoto

Author Affiliations +

Journal of Biomedical Optics, Vol. 18, Issue 9, 096009 (September 2013). https://doi.org/10.1117/1.JBO.18.9.096009

Abstract

We develop a coherent anti-Stokes Raman scattering (CARS) microscopy system equipped with a tunable picosecond laser for high-speed wavelength scanning. An acousto-optic tunable filter (AOTF) is integrated in the laser cavity to enable wavelength scanning by varying the radio frequency waves applied to the AOTF crystal. An end mirror attached on a piezoelectric actuator and a pair of parallel plates driven by galvanometer motors are also introduced into the cavity to compensate for changes in the cavity length during wavelength scanning to allow synchronization with another picosecond laser. We demonstrate fast spectral imaging of 3T3-L1 adipocytes every 5 cm ⁻¹ in the Raman spectral region around 2850 cm⁻¹ with an image acquisition time of 120 ms. We also demonstrate fast switching of Raman shifts between 2100 and 2850 cm ⁻¹ , corresponding to CD ₂ symmetric stretching and CH ₂ symmetric stretching vibrations, respectively. The fast-switching CARS images reveal different locations of recrystallized deuterated and nondeuterated stearic acid.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Harsono Cahyadi, Junichi Iwatsuka, Takeo Minamikawa, Hirohiko Niioka, Tsutomu Araki, and Mamoru Hashimoto "Fast spectral coherent anti-Stokes Raman scattering microscopy with high-speed tunable picosecond laser," Journal of Biomedical Optics 18(9), 096009 (6 September 2013). https://doi.org/10.1117/1.JBO.18.9.096009

Published: 6 September 2013

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Cited by 19 scholarly publications.

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KEYWORDS

Microscopy

Raman spectroscopy

CARS tomography

Picosecond phenomena

Tunable lasers

Imaging systems

Signal detection

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