Simultaneous imaging of cellular morphology and multiple biomarkers using an acousto-optic tunable filter–based bright field microscope

Elliot S. Wachman; Stanley J. Geyer; Joel M. Recht; Jon D. Ward; Bill G. Zhang; Murray K. Reed; Christopher N. Pannell

doi:10.1117/1.JBO.19.5.056006

9 May 2014 Simultaneous imaging of cellular morphology and multiple biomarkers using an acousto-optic tunable filter–based bright field microscope

Elliot S. Wachman, Stanley J. Geyer, Joel M. Recht, Jon D. Ward, Bill G. Zhang, Murray K. Reed, Christopher N. Pannell

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Journal of Biomedical Optics, Vol. 19, Issue 5, 056006 (May 2014). https://doi.org/10.1117/1.JBO.19.5.056006

Abstract

An acousto-optic tunable filter (AOTF)-based multispectral imaging microscope system allows the combination of cellular morphology and multiple biomarker stainings on a single microscope slide. We describe advances in AOTF technology that have greatly improved spectral purity, field uniformity, and image quality. A multispectral imaging bright field microscope using these advances demonstrates pathology results that have great potential for clinical use.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Elliot S. Wachman, Stanley J. Geyer, Joel M. Recht, Jon D. Ward, Bill G. Zhang, Murray K. Reed, and Christopher N. Pannell "Simultaneous imaging of cellular morphology and multiple biomarkers using an acousto-optic tunable filter–based bright field microscope," Journal of Biomedical Optics 19(5), 056006 (9 May 2014). https://doi.org/10.1117/1.JBO.19.5.056006

Published: 9 May 2014

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