In vivo measurement of tissue oxygenation by time-resolved luminescence spectroscopy: advantageous properties of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate

Veronika Huntosova; Sandrine Gay; Patrycja M. Nowak-Sliwinska; Senthil  K. Rajendran; Matthieu Zellweger; Hubert van den Bergh; Georges Wagnières

doi:10.1117/1.JBO.19.7.077004

18 July 2014 In vivo measurement of tissue oxygenation by time-resolved luminescence spectroscopy: advantageous properties of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate

Veronika Huntosova, Sandrine Gay, Patrycja M. Nowak-Sliwinska, Senthil K. Rajendran, Matthieu Zellweger, Hubert van den Bergh, Georges Wagnières

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Journal of Biomedical Optics, Vol. 19, Issue 7, 077004 (July 2014). https://doi.org/10.1117/1.JBO.19.7.077004

Abstract

Measuring tissue oxygenation in vivo is of interest in fundamental biological as well as medical applications. One minimally invasive approach to assess the oxygen partial pressure in tissue (pO₂) is to measure the oxygen-dependent luminescence lifetime of molecular probes. The relation between tissue pO₂ and the probes’ luminescence lifetime is governed by the Stern-Volmer equation. Unfortunately, virtually all oxygen-sensitive probes based on this principle induce some degree of phototoxicity. For that reason, we studied the oxygen sensitivity and phototoxicity of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate [Ru(Phen)] using a dedicated optical fiber–based, time-resolved spectrometer in the chicken embryo chorioallantoic membrane. We demonstrated that, after intravenous injection, Ru(Phen)’s luminescence lifetime presents an easily detectable pO₂ dependence at a low drug dose (1 mg/kg) and low fluence (120 mJ/cm₂ at 470 nm). The phototoxic threshold was found to be at 10 J/cm² with the same wavelength and drug dose, i.e., about two orders of magnitude larger than the fluence necessary to perform a pO₂ measurement. Finally, an illustrative application of this pO₂ measurement approach in a hypoxic tumor environment is presented.

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Veronika Huntosova, Sandrine Gay, Patrycja M. Nowak-Sliwinska, Senthil K. Rajendran, Matthieu Zellweger, Hubert van den Bergh, and Georges Wagnières "In vivo measurement of tissue oxygenation by time-resolved luminescence spectroscopy: advantageous properties of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate," Journal of Biomedical Optics 19(7), 077004 (18 July 2014). https://doi.org/10.1117/1.JBO.19.7.077004

Published: 18 July 2014

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