Quantification of in vitro mesenchymal stem cell invasion into tumor spheroids using selective plane illumination microscopy

Svenja Rühland; Alexandra Wechselberger; Christine Spitzweg; Ralf Huss; Peter J. Nelson; Hartmann Harz

doi:10.1117/1.JBO.20.4.040501

3 April 2015 Quantification of in vitro mesenchymal stem cell invasion into tumor spheroids using selective plane illumination microscopy

Svenja Rühland, Alexandra Wechselberger, Christine Spitzweg, Ralf Huss, Peter J. Nelson, Hartmann Harz

Author Affiliations +

Journal of Biomedical Optics, Vol. 20, Issue 4, 040501 (April 2015). https://doi.org/10.1117/1.JBO.20.4.040501

Abstract

Mesenchymal stem cell (MSC) homing and integration into tumors are under evaluation for clinical application. This approach requires the identification of conditions for optimal tumor invasion. We describe a tool for the in vitro comparison of parameters influencing invasion. Human MSC added to experimental tumor spheroids variably migrates toward the center of the structure. To determine MSC distribution inside the three-dimensional specimen, spatial analysis was performed using selective plane illumination microscopy. A standardized method to quantify and compare the invasion potential of variably treated MSC into experimental tumor environments allows efficient screening for optimizing conditions.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Citation Download Citation

Svenja Rühland, Alexandra Wechselberger, Christine Spitzweg, Ralf Huss, Peter J. Nelson, and Hartmann Harz "Quantification of in vitro mesenchymal stem cell invasion into tumor spheroids using selective plane illumination microscopy," Journal of Biomedical Optics 20(4), 040501 (3 April 2015). https://doi.org/10.1117/1.JBO.20.4.040501

Published: 3 April 2015

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
3 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY