Stimulus-evoked outer segment changes in rod photoreceptors

Xiaohui Zhao; Damber Thapa; Benquan Wang; Yiming Lu; Shaoyan Gai; Xincheng Yao

doi:10.1117/1.JBO.21.6.065006

23 June 2016 Stimulus-evoked outer segment changes in rod photoreceptors

Xiaohui Zhao, Damber Thapa, Benquan Wang, Yiming Lu, Shaoyan Gai, Xincheng Yao

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Journal of Biomedical Optics, Vol. 21, Issue 6, 065006 (June 2016). https://doi.org/10.1117/1.JBO.21.6.065006

Abstract

Rod-dominated transient retinal phototropism (TRP) has been recently observed in freshly isolated mouse and frog retinas. Comparative confocal microscopy and optical coherence tomography revealed that the TRP was predominantly elicited from the rod outer segment (OS). However, the biophysical mechanism of rod OS dynamics is still unknown. Mouse and frog retinal slices, which displayed a cross-section of retinal photoreceptors and other functional layers, were used to test the effect of light stimulation on rod OSs. Time-lapse microscopy revealed stimulus-evoked conformational changes of rod OSs. In the center of the stimulated region, the length of the rod OS shrunk, while in the peripheral region, the rod OS swung toward the center region. Our experimental observation and theoretical analysis suggest that the TRP may reflect unbalanced rod disc-shape changes due to localized visible light stimulation.

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Xiaohui Zhao, Damber Thapa, Benquan Wang, Yiming Lu, Shaoyan Gai, and Xincheng Yao "Stimulus-evoked outer segment changes in rod photoreceptors," Journal of Biomedical Optics 21(6), 065006 (23 June 2016). https://doi.org/10.1117/1.JBO.21.6.065006

Published: 23 June 2016

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