Fiber-based 1150-nm femtosecond laser source for the minimally invasive harmonic generation microscopy

Jing-Yu Huang; Lun-Zhang Guo; Jing-Zun Wang; Tse-Chung Li; Hsin-Jung Lee; Po-Kai Chiu; Lung-Han Peng; Tzu-Ming Liu

doi:10.1117/1.JBO.22.3.036008

8 March 2017 Fiber-based 1150-nm femtosecond laser source for the minimally invasive harmonic generation microscopy

Jing-Yu Huang, Lun-Zhang Guo, Jing-Zun Wang, Tse-Chung Li, Hsin-Jung Lee, Po-Kai Chiu, Lung-Han Peng, Tzu-Ming Liu

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Journal of Biomedical Optics, Vol. 22, Issue 3, 036008 (March 2017). https://doi.org/10.1117/1.JBO.22.3.036008

Abstract

Harmonic generation microscopy (HGM) has become one unique tool of optical virtual biopsy for the diagnosis of cancer and the in vivo cytometry of leukocytes. Without labeling, HGM can reveal the submicron features of tissues and cells in vivo. For deep imaging depth and minimal invasiveness, people commonly adopt 1100- to 1300-nm femtosecond laser sources. However, those lasers are typically based on bulky oscillators whose performances are sensitive to environmental conditions. We demonstrate a fiber-based 1150-nm femtosecond laser source, with 6.5-nJ pulse energy, 86-fs pulse width, and 11.25-MHz pulse repetition rate. It was obtained by a bismuth borate or magnesium-doped periodically poled lithium niobate (MgO:PPLN) mediated frequency doubling of the 2300-nm solitons, generated from an excitation of 1550-nm femtosecond pulses on a large mode area photonic crystal fiber. Combined with a home-built laser scanned microscope and a tailor-made frame grabber, we achieve a pulse-per-pixel HGM imaging in vivo at a 30-Hz frame rate. This integrated solution has the potential to be developed as a stable HGM system for routine clinical use.

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Jing-Yu Huang, Lun-Zhang Guo, Jing-Zun Wang, Tse-Chung Li, Hsin-Jung Lee, Po-Kai Chiu, Lung-Han Peng, and Tzu-Ming Liu "Fiber-based 1150-nm femtosecond laser source for the minimally invasive harmonic generation microscopy," Journal of Biomedical Optics 22(3), 036008 (8 March 2017). https://doi.org/10.1117/1.JBO.22.3.036008

Received: 18 September 2016; Accepted: 3 February 2017; Published: 8 March 2017

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