Automated classification of cell morphology by coherence-controlled holographic microscopy

Lenka Strbkova; Daniel Zicha; Pavel Vesely M.D.; Radim Chmelik

doi:10.1117/1.JBO.22.8.086008

23 August 2017 Automated classification of cell morphology by coherence-controlled holographic microscopy

Lenka Strbkova, Daniel Zicha, Pavel Vesely M.D., Radim Chmelik

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Journal of Biomedical Optics, Vol. 22, Issue 8, 086008 (August 2017). https://doi.org/10.1117/1.JBO.22.8.086008

Abstract

In the last few years, classification of cells by machine learning has become frequently used in biology. However, most of the approaches are based on morphometric (MO) features, which are not quantitative in terms of cell mass. This may result in poor classification accuracy. Here, we study the potential contribution of coherence-controlled holographic microscopy enabling quantitative phase imaging for the classification of cell morphologies. We compare our approach with the commonly used method based on MO features. We tested both classification approaches in an experiment with nutritionally deprived cancer tissue cells, while employing several supervised machine learning algorithms. Most of the classifiers provided higher performance when quantitative phase features were employed. Based on the results, it can be concluded that the quantitative phase features played an important role in improving the performance of the classification. The methodology could be valuable help in refining the monitoring of live cells in an automated fashion. We believe that coherence-controlled holographic microscopy, as a tool for quantitative phase imaging, offers all preconditions for the accurate automated analysis of live cell behavior while enabling noninvasive label-free imaging with sufficient contrast and high-spatiotemporal phase sensitivity.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Lenka Strbkova, Daniel Zicha, Pavel Vesely M.D., and Radim Chmelik "Automated classification of cell morphology by coherence-controlled holographic microscopy," Journal of Biomedical Optics 22(8), 086008 (23 August 2017). https://doi.org/10.1117/1.JBO.22.8.086008

Received: 5 May 2017; Accepted: 28 July 2017; Published: 23 August 2017

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