Label-free reflectance hyperspectral imaging for tumor margin assessment: a pilot study on surgical specimens of cancer patients

Baowei Fei; Guolan Lu; Xu Wang; Hongzheng Zhang; James V. Little M.D.; Mihir R. Patel; Christopher C. Griffith; Mark W. El-Diery; Amy Y. Chen

doi:10.1117/1.JBO.22.8.086009

28 August 2017 Label-free reflectance hyperspectral imaging for tumor margin assessment: a pilot study on surgical specimens of cancer patients

Baowei Fei, Guolan Lu, Xu Wang, Hongzheng Zhang, James V. Little M.D., Mihir R. Patel, Christopher C. Griffith, Mark W. El-Diery, Amy Y. Chen

Author Affiliations +

Journal of Biomedical Optics, Vol. 22, Issue 8, 086009 (August 2017). https://doi.org/10.1117/1.JBO.22.8.086009

Abstract

A label-free, hyperspectral imaging (HSI) approach has been proposed for tumor margin assessment. HSI data, i.e., hypercube (x,y,λ), consist of a series of high-resolution images of the same field of view that are acquired at different wavelengths. Every pixel on an HSI image has an optical spectrum. In this pilot clinical study, a pipeline of a machine-learning-based quantification method for HSI data was implemented and evaluated in patient specimens. Spectral features from HSI data were used for the classification of cancer and normal tissue. Surgical tissue specimens were collected from 16 human patients who underwent head and neck (H&N) cancer surgery. HSI, autofluorescence images, and fluorescence images with 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose (2-NBDG) and proflavine were acquired from each specimen. Digitized histologic slides were examined by an H&N pathologist. The HSI and classification method were able to distinguish between cancer and normal tissue from the oral cavity with an average accuracy of 90%±8%, sensitivity of 89%±9%, and specificity of 91%±6%. For tissue specimens from the thyroid, the method achieved an average accuracy of 94%±6%, sensitivity of 94%±6%, and specificity of 95%±6%. HSI outperformed autofluorescence imaging or fluorescence imaging with vital dye (2-NBDG or proflavine). This study demonstrated the feasibility of label-free, HSI for tumor margin assessment in surgical tissue specimens of H&N cancer patients. Further development of the HSI technology is warranted for its application in image-guided surgery.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Citation Download Citation

Baowei Fei, Guolan Lu, Xu Wang, Hongzheng Zhang, James V. Little M.D., Mihir R. Patel, Christopher C. Griffith, Mark W. El-Diery, and Amy Y. Chen "Label-free reflectance hyperspectral imaging for tumor margin assessment: a pilot study on surgical specimens of cancer patients," Journal of Biomedical Optics 22(8), 086009 (28 August 2017). https://doi.org/10.1117/1.JBO.22.8.086009

Received: 9 May 2017; Accepted: 2 August 2017; Published: 28 August 2017

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY