CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 23 > Issue 12 > Article
Open Access
6 December 2018 Ultrafast laser-scanning optical resolution photoacoustic microscopy at up to 2 million A-lines per second
Thomas John Allen, Josh Spurrell, Martin O. Berendt, Olumide Ogunlade, Shaif U. Alam, Edward Z. Zhang, David J. Richardson, Paul C. Beard
Author Affiliations +
Funded by: Engineering and Physical Sciences Research Council (EPSRC), European Union project FAMOS, King’s College London, University College London
Journal of Biomedical Optics, Vol. 23, Issue 12, 126502 (December 2018). https://doi.org/10.1117/1.JBO.23.12.126502
Abstract
The imaging speed of optical resolution photoacoustic microscopy (OR-PAM) using pulsed excitation is fundamentally limited by the range ambiguity condition, which defines the maximum laser pulse repetition frequency (PRF). To operate at this theoretical upper limit and maximize acquisition speed, a custom-built fiber laser capable of operating at a PRF of up to 2 MHz was combined with a fast laser scanning optical OR-PAM system based on a stationary fiber-optic ultrasound sensor. A large area (10  mm  ×  10  mm) of the mouse ear was imaged within 8 s, when acquiring 16 million A-lines and operating the laser at a PRF of 2 MHz. This corresponds to a factor of four improvement in imaging speed compared to the fastest OR-PAM system previously reported. The ability to operate at high-imaging frame rates also allows the capture of hemodynamic events such as blood flow. It is considered that this system offers opportunities for high throughput imaging and visualizing dynamic physiological events using OR-PAM.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Citation Download Citation
Thomas John Allen, Josh Spurrell, Martin O. Berendt, Olumide Ogunlade, Shaif U. Alam, Edward Z. Zhang, David J. Richardson, and Paul C. Beard "Ultrafast laser-scanning optical resolution photoacoustic microscopy at up to 2 million A-lines per second," Journal of Biomedical Optics 23(12), 126502 (6 December 2018). https://doi.org/10.1117/1.JBO.23.12.126502
Received: 15 August 2018; Accepted: 26 October 2018; Published: 6 December 2018
JOURNAL ARTICLE
 9 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 23 scholarly publications.
Advertisement
Advertisement
KEYWORDS
Video
Fiber lasers
Ear
Imaging systems
Optical resolution
Photoacoustic microscopy
Photoacoustic spectroscopy
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top