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24 September 2018 Nonlinear relation between concentration and fluorescence emission of protoporphyrin IX in calibrated phantoms
Laure Alston, David Rousseau, Mathieu Hébert, Laurent Mahieu-Williame, Bruno Montcel
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Journal of Biomedical Optics, Vol. 23, Issue 9, 097002 (September 2018). https://doi.org/10.1117/1.JBO.23.9.097002
Abstract
5-ALA-induced protoporphyrin IX (PpIX) has shown its relevance in medical assisting techniques, notably in the detection of glioma (brain tumors). Validation of instruments on phantoms is mandatory and a standardization procedure has recently been proposed. This procedure yields phantoms recipes to realize a linear relationship between PpIX concentration and fluorescence emission intensity. The present study puts forward phantoms where this linear relationship cannot be used. We propose a model that considers two states of PpIX, corresponding to two different aggregates of PpIX, with fluorescence spectra peaking at 634 and 620 nm, respectively. We characterize the influence of these two states on PpIX fluorescence emission spectra in phantoms with steady concentration of PpIX and various microenvironment parameters (surfactant, Intralipid or bovine blood concentration, and pH). We show that, with fixed PpIX concentration, a modification of the microenvironment induces a variation of the emitted spectrum, notably a shift in its central wavelength. We show that this modification reveals a variation of proportions of the two states. This establishes phantom microenvironment regimes where the usual single state model is biased while a linear combination of the two spectra enables accurate recovering of any measured spectra.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2018/$25.00 © 2018 SPIE
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Laure Alston, David Rousseau, Mathieu Hébert, Laurent Mahieu-Williame, and Bruno Montcel "Nonlinear relation between concentration and fluorescence emission of protoporphyrin IX in calibrated phantoms," Journal of Biomedical Optics 23(9), 097002 (24 September 2018). https://doi.org/10.1117/1.JBO.23.9.097002
Received: 12 December 2017; Accepted: 5 September 2018; Published: 24 September 2018
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KEYWORDS
Luminescence
Blood
Tissues
Calibration
Linear filtering
Tumors
Fluorescence spectroscopy
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