Aberration correction method based on double-helix point spread function

Zhaojun Wang; Yanan Cai; Yansheng Liang; Dan Dan; Baoli Yao; Ming Lei

doi:10.1117/1.JBO.24.3.031005

4 September 2018 Aberration correction method based on double-helix point spread function

Zhaojun Wang, Yanan Cai, Yansheng Liang, Dan Dan, Baoli Yao, Ming Lei

Author Affiliations +

Funded by: National Key Research and Development Program of China, National Natural Science Foundation of China (NSFC), Natural Science Foundation of China (NSFC), Natural Science Foundation of China

Journal of Biomedical Optics, Vol. 24, Issue 3, 031005 (September 2018). https://doi.org/10.1117/1.JBO.24.3.031005

Abstract

Point spread function (PSF) engineering has met with lots of interest in various optical imaging techniques, including super-resolution microscopy, microparticle tracking, and extended depth-of-field microscopy. The intensity distributions of the modified PSFs often suffer from deteriorations caused by system aberrations, which greatly degrade the image contrast, resolution, or localization precision. We present an aberration correction method using a spiral-phase-based double-helix PSF as an aberration indicator, which is sensitive and quantitatively correlated to the spherical aberration, coma, and astigmatism. Superior to the routine iteration-based correction methods, the presented approach is iteration-free and the aberration coefficients can be directly calculated with the measured parameters, relieving the computing burden. The validity of the method is verified by both examining the intensity distribution of the conventional Gaussian PSF in three dimensions and observing muntjac skin fibroblast cells. This iteration-free correction method has a potential application in PSF engineering systems equipped with a spatial light modulator.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Zhaojun Wang, Yanan Cai, Yansheng Liang, Dan Dan, Baoli Yao, and Ming Lei "Aberration correction method based on double-helix point spread function," Journal of Biomedical Optics 24(3), 031005 (4 September 2018). https://doi.org/10.1117/1.JBO.24.3.031005

Received: 23 June 2018; Accepted: 16 August 2018; Published: 4 September 2018

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