Mobile-phone-based Rheinberg microscope with a light-emitting diode array

Yuma Ogasawara; Ryo Sugimoto; Ryoji Maruyama; Hidenobu Arimoto; Yosuke Tamada; Wataru Watanabe

doi:10.1117/1.JBO.24.3.031007

24 September 2018 Mobile-phone-based Rheinberg microscope with a light-emitting diode array

Yuma Ogasawara, Ryo Sugimoto, Ryoji Maruyama, Hidenobu Arimoto, Yosuke Tamada, Wataru Watanabe

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Funded by: JSPS KAKENHI

Journal of Biomedical Optics, Vol. 24, Issue 3, 031007 (September 2018). https://doi.org/10.1117/1.JBO.24.3.031007

Abstract

Mobile phone technology has led to implementation of portable and inexpensive microscopes. Light-emitting diode (LED) array microscopes support various multicontrast imaging by flexible illumination patterns of the LED array that can be achieved without changing the optical components of the microscope. Here, we demonstrate a mobile-phone-based LED array microscope to realize multimodal imaging with bright-field, dark-field, differential phase-contrast, and Rheinberg illuminations using as few as 37 LED bulbs. Using this microscope, we obtained high-contrast images of living cells. Furthermore, by changing the color combinations of Rheinberg illumination, we were able to obtain images of living chromatic structures with enhanced or diminished contrast. This technique is expected to be a foundation for high-contrast microscopy used in modern field studies.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Yuma Ogasawara, Ryo Sugimoto, Ryoji Maruyama, Hidenobu Arimoto, Yosuke Tamada, and Wataru Watanabe "Mobile-phone-based Rheinberg microscope with a light-emitting diode array," Journal of Biomedical Optics 24(3), 031007 (24 September 2018). https://doi.org/10.1117/1.JBO.24.3.031007

Received: 1 July 2018; Accepted: 28 August 2018; Published: 24 September 2018

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