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11 July 2023 Effect of matrix properties on transmission and reflectance mode division-of-focal-plane Stokes polarimetry
Leanne E. Iannucci, Matthew B. Riak, Ethan Meitz, Matthew R. Bersi, Viktor Gruev, Spencer P. Lake
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Journal of Biomedical Optics, Vol. 28, Issue 10, 102902 (July 2023). https://doi.org/10.1117/1.JBO.28.10.102902
Abstract

Significance

Division-of-focal-plane Stokes polarimetry is emerging as a powerful tool for the microstructural characterization of soft tissues. How individual extracellular matrix (ECM) properties influence polarimetric signals in reflectance or transmission modes of quantitative polarized light imaging (QPLI) is not well understood.

Aim

We aimed to investigate how ECM properties affect outcomes obtained from division-of-focal-plane polarimetric imaging in reflectance or transmission modes.

Approach

Tunable collagen gel phantoms were used to modulate ECM properties of anisotropy, collagen density, crosslinking, and absorber density; the effects of degree of linear polarization (DoLP) and angle of polarization (AoP) on polarimetry outcomes were assessed. A model biological tissue (i.e., bovine tendon) was similarly imaged and evaluated using both reflectance and transmission modes.

Results

Reflectance QPLI resulted in decreased DoLP compared with transmission mode. A 90 deg shift in AoP was observed between modes but yielded similar spatial patterns. Collagen density had the largest effect on outcomes besides anisotropy in both imaging modes.

Conclusions

Both imaging modes were sufficiently sensitive to detect structural anisotropy differences in gels of varying fiber alignment. Conclusions drawn from phantom experiments should carry over when interpreting data from more complex tissues and can help provide context for interpretation of other Stokes polarimetry data.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Leanne E. Iannucci, Matthew B. Riak, Ethan Meitz, Matthew R. Bersi, Viktor Gruev, and Spencer P. Lake "Effect of matrix properties on transmission and reflectance mode division-of-focal-plane Stokes polarimetry," Journal of Biomedical Optics 28(10), 102902 (11 July 2023). https://doi.org/10.1117/1.JBO.28.10.102902
Received: 5 March 2023; Accepted: 23 May 2023; Published: 11 July 2023
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KEYWORDS
Collagen
Reflectivity
Tissues
Polarimetry
Biological imaging
Matrices
Optical properties
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