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10 February 2023 Cable-free brain imaging for multiple free-moving animals with miniature wireless microscopes
Yangzhen Wang, Zhongtian Ma, Wenzhao Li, Feng Su, Chong Wang, Wei Xiong, Changhui Li, Chen Zhang
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Journal of Biomedical Optics, Vol. 28, Issue 2, 026503 (February 2023). https://doi.org/10.1117/1.JBO.28.2.026503
Abstract

Significance

Although several miniature microscope systems have been developed to allow researchers to image brain neuron activities of free moving rodents, they generally require a long cable connecting to the miniature microscope. It not only limits the behavior of the animal, but also makes it challenging to study multiple animals simultaneously.

Aim

The aim of this work is to develop a fully wireless miniature microscope that would break constraints from the connecting cables so that the animals could move completely freely, allowing neuroscience researchers to study more of animals’ behaviors simultaneously, such as social behavior.

Approach

We present a wireless mini-microscope (wScope) that enables simultaneously real-time brain imaging preview from multiple free-moving animals. The wScope has a mass of 2.7 g and a maximum frame rate of 25 Hz at 750 μm × 450 μm field of view with 1.8-μm resolution.

Results

The performance of the wScope is validated via real-time imaging of the cerebral blood flow and the activity of neurons in the primary visual cortex (V1) of different mice.

Conclusions

The wScope provides a powerful tool for brain imaging of multiple free moving animals in their much larger spaces and more naturalistic environments.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Yangzhen Wang, Zhongtian Ma, Wenzhao Li, Feng Su, Chong Wang, Wei Xiong, Changhui Li, and Chen Zhang "Cable-free brain imaging for multiple free-moving animals with miniature wireless microscopes," Journal of Biomedical Optics 28(2), 026503 (10 February 2023). https://doi.org/10.1117/1.JBO.28.2.026503
Received: 12 October 2022; Accepted: 20 January 2023; Published: 10 February 2023
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Cited by 2 scholarly publications.
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KEYWORDS
Animals
Microscopes
Imaging systems
Brain imaging
Neurons
Batteries
Image quality
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