Quantitative phase imaging techniques for measuring scattering properties of cells and tissues: a review—part I

Neha Goswami; Mark A. Anastasio; Gabriel Popescu

doi:10.1117/1.JBO.29.S2.S22713

18 July 2024 Quantitative phase imaging techniques for measuring scattering properties of cells and tissues: a review—part I

Neha Goswami, Mark A. Anastasio, Gabriel Popescu

Author Affiliations +

Journal of Biomedical Optics, Vol. 29, Issue S2, S22713 (July 2024). https://doi.org/10.1117/1.JBO.29.S2.S22713

Abstract

Significance

Quantitative phase imaging (QPI) techniques offer intrinsic information about the sample of interest in a label-free, noninvasive manner and have an enormous potential for wide biomedical applications with negligible perturbations to the natural state of the sample in vitro.

Aim

We aim to present an in-depth review of the scattering formulation of light–matter interactions as applied to biological samples such as cells and tissues, discuss the relevant quantitative phase measurement techniques, and present a summary of various reported applications.

Approach

We start with scattering theory and scattering properties of biological samples followed by an exploration of various microscopy configurations for 2D QPI for measurement of structure and dynamics.

Results

We reviewed 157 publications and presented a range of QPI techniques and discussed suitable applications for each. We also presented the theoretical frameworks for phase reconstruction associated with the discussed techniques and highlighted their domains of validity.

Conclusions

We provide detailed theoretical as well as system-level information for a wide range of QPI techniques. Our study can serve as a guideline for new researchers looking for an exhaustive literature review of QPI methods and relevant applications.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Neha Goswami, Mark A. Anastasio, and Gabriel Popescu "Quantitative phase imaging techniques for measuring scattering properties of cells and tissues: a review—part I," Journal of Biomedical Optics 29(S2), S22713 (18 July 2024). https://doi.org/10.1117/1.JBO.29.S2.S22713

Received: 29 February 2024; Accepted: 20 May 2024; Published: 18 July 2024

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KEYWORDS

Scattering

Tissues

Phase measurement

Phase imaging

Refractive index

Light scattering

Biological samples

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