Detection of glutamate in the eye by Raman spectroscopy

Alvin Katz; Erik F. Kruger; Glenn Minko; C. H. Liu; Richard B. Rosen M.D.; Robert R. Alfano

doi:10.1117/1.1559726

1 April 2003 Detection of glutamate in the eye by Raman spectroscopy

Alvin Katz, Erik F. Kruger, Glenn Minko, C. H. Liu, Richard B. Rosen M.D., Robert R. Alfano

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Journal of Biomedical Optics, Vol. 8, Issue 2, (April 2003). https://doi.org/10.1117/1.1559726

Raman spectroscopy is used to detect glutamate in the eye. Glutamate, a by-product of nerve cell death, is an indicator of glaucoma and diabetic retinopathy. The Raman spectra of ex vivo whole porcine eyes and individual components (lens, cornea, vitreous) are measured and characterized. Monosodium glutamate is injected into the eyes to simulate disease conditions, and the contribution to the Raman spectrum due to the presence of glutamate is identified. The Raman spectra from the native eye is dominated by vibrational modes from proteins in the lens. An optical system is designed to optimize collection of signal from the vitreous, where the glutamate is located, and reduce the Raman from the lens. Two vibrational fingerprints of monosodium glutamate are detected at 1369 and 1422 cm^–1, although the concentrations are much above physiological concentrations.

©(2003) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Alvin Katz, Erik F. Kruger, Glenn Minko, C. H. Liu, Richard B. Rosen M.D., and Robert R. Alfano "Detection of glutamate in the eye by Raman spectroscopy," Journal of Biomedical Optics 8(2), (1 April 2003). https://doi.org/10.1117/1.1559726

Published: 1 April 2003

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