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1 March 2004 Mitochondria in tumor cells studied by laser scanning confocal microscopy
Anna Maria Villa, Silvia M. Doglia
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Journal of Biomedical Optics, Vol. 9, Issue 2, (March 2004). https://doi.org/10.1117/1.1646414
We present here a confocal fluorescence microscopy study of mitochondria in sensitive and resistant carcinoma cells by using two potentiometric probes of mitochondria, rhodamine 123 (R123) and dimethylaminostyryl-methylpyridiniumiodine. We have found that active mitochondria in sensitive MCF-7 and multidrug resistant MCF-7/DX carcinoma cells are very different in localization and morphology. In sensitive cells active mitochondria are found in the perinuclear region, whereas in the multidrug resistance (MDR) subline they are confined to the cell periphery. Interestingly, the MDR revertant verapamil has been found to restore in MCF-7/DX cells the same pattern of active mitochondria seen in sensitive cells. We have also studied R123 in human lung carcinoma A549 cells, which display a low responsivity to doxorubicin, and overexpress the lung resistance-related protein. In addition to perinuclear mitochondria, peripheral mitochondria with weaker fluorescence can be seen in this cell line. Interestingly, in the two examined carcinoma lines we have been able to recognize by image analysis a common new star-lobed morphology. Our results indicate that in resistant carcinoma cells two populations of mitochondria coexist with different localization, morphology, and activity.
©(2004) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Anna Maria Villa and Silvia M. Doglia "Mitochondria in tumor cells studied by laser scanning confocal microscopy," Journal of Biomedical Optics 9(2), (1 March 2004). https://doi.org/10.1117/1.1646414
Published: 1 March 2004
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KEYWORDS
Luminescence
Resistance
Tumors
Confocal microscopy
Calcium
Image analysis
Optical filters
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