Spiral laser scanning photoacoustic microscopy for functional brain imaging in rats

Mohsin Zafar; Laura Stone McGuire; Seyed Mohsen Ranjbaran; James I. Matchynski; Rayyan Manwar; Alana C. C. Conti; Shane A. Perrine; Kamran Avanaki

doi:10.1117/1.NPh.11.1.015007

9 February 2024 Spiral laser scanning photoacoustic microscopy for functional brain imaging in rats

Mohsin Zafar, Laura Stone McGuire, Seyed Mohsen Ranjbaran, James I. Matchynski, Rayyan Manwar, Alana C. C. Conti, Shane A. Perrine, Kamran Avanaki

Author Affiliations +

Neurophotonics, Vol. 11, Issue 1, 015007 (February 2024). https://doi.org/10.1117/1.NPh.11.1.015007

Abstract

Significance

There are many neuroscience questions that can be answered by a high-resolution functional brain imaging system. Such a system would require the capability to visualize vasculature and measure neural activity by imaging the entire brain continually and in rapid succession in order to capture hemodynamic changes. Utilizing optical excitation and acoustic detection, photoacoustic technology enables label-free quantification of changes in endogenous chromophores, such as oxyhemoglobin, deoxyhemoglobin, and total hemoglobin.

Aim

Our aim was to develop a sufficiently high-resolution, fast frame-rate, and wide field-of-view (FOV) photoacoustic microscopy (PAM) system for the purpose of imaging vasculature and hemodynamics in a rat brain.

Approach

Although the most PA microscopy systems use raster scanning (or less commonly Lissajous scanning), we have developed a simple-to-implement laser scanning optical resolution PAM system with spiral scanning (which we have named “spiral laser scanning photoacoustic microscopy” or sLS-PAM) to acquire an 18 mm diameter image at fast frame rate (more than 1 fps). Such a system is designed to permit continuous rat brain imaging without the introduction of photobleaching artifacts.

Conclusion

We demonstrated the functional imaging capability of the sLS-PAM system by imaging cerebral hemodynamics in response to whisker and electrical stimulation and used it for vascular imaging of a modeled brain injury. We believe that we have demonstrated the development of a simple-to-implement PAM system, which could become an affordable functional neuroimaging tool for researchers.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Mohsin Zafar, Laura Stone McGuire, Seyed Mohsen Ranjbaran, James I. Matchynski, Rayyan Manwar, Alana C. C. Conti, Shane A. Perrine, and Kamran Avanaki "Spiral laser scanning photoacoustic microscopy for functional brain imaging in rats," Neurophotonics 11(1), 015007 (9 February 2024). https://doi.org/10.1117/1.NPh.11.1.015007

Received: 14 December 2022; Accepted: 8 January 2024; Published: 9 February 2024

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KEYWORDS

Brain

Imaging systems

Neuroimaging

Transducers

Laser scanners

Photoacoustic microscopy

Hemodynamics

Significance

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