In this paper we have studied effect of a hyperosmotic optical clearing agent (OCA), such as polyethylene glycol,
on the fluorescence intensity from a target located in subcutaneous area in the model experiments. As a
fluorescence agent the nanocomposite including gold nanorods with hematophorphyrin was used. The remitted
fluorescent signal traveling to the tissue surface was monitored over time as the tissue was treated with the OCA.
The detected fluorescent signal increased as the scattering in tissue samples was substantially reduced. The study
has shown how OCA can be used to improve the detected signal at localization of subcutaneous target tissue at
the photothermal or photodynamic therapy. Immersion clearing of skin can be also useful for improvement of
laser exposure efficiency due to the increasing of light penetration depth.
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