Ms. Amanda C. Fraley Profile

Amanda C. Fraley

Research Assistant at Univ of Tennessee

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Proceedings Article | 14 June 2004 Paper

Bioluminescent bioreporter sensing of foodborne toxins

Amanda Fraley, Steven Ripp, Gary Sayler

Proceedings Volume 5329, (2004) https://doi.org/10.1117/12.529272

KEYWORDS: Bioluminescence, Biosensing, Biosensors, Proteins, Toxicity, Bacteria, Microorganisms, Industrial chemicals, Visible radiation, Ocean optics

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Histamine is the primary etiological agent in the foodborne disease scombrotoxicosis, one of the most common food toxicities related to fish consumption. Procedures for detecting histamine in fish products are available, but are often too expensive or too complex for routine use. As an alternative, a bacterial bioluminescent bioreporter has been constructed to develop a biosensor system that autonomously responds to low levels of histamine. The bioreporter contains a promoterless Photorhabdus luminescens lux operon (luxCDABE) fused with the Vibrio anguillarum angR regulatory gene promoter of the anguibactin biosynthetic operon. The bioreporter emitted 1.46 times more bioluminescence than background, 30 minutes after the addition of 100mM histamine. However, specificity was not optimal, as this biosensor generated significant bioluminescence in the presence of L-proline and L-histidine. As a means towards improving histamine specificity, the promoter region of a histamine oxidase gene from Arthrobacter globiformis was cloned upstream of the promotorless lux operon from Photorhabdus luminescens. This recently constructed whole-cell, lux-based bioluminescent bioreporter is currently being tested for optimal performance in the presence of histamine in order to provide a rapid, simple, and inexpensive model sensor for the detection of foodborne toxins.

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