KEYWORDS: Ultrasonography, Brain, In vivo imaging, Acoustics, In vitro testing, Potassium, Modulation, Molecular mechanisms, Transducers, Chemical elements
Low intensity focused ultrasound (LIFU) is now being considered as a noninvasive brain therapy for clinical
applications. We maintain that LIFU can efficiently deliver energy from outside the skull to target specific brain regions,
effecting localized neuromodulation. However, the underlying molecular mechanisms that drive this LIFU-induced
neuromodulation are not well-defined due, in part, to our lack of understanding of how particular sets of LIFU delivery
parameters affect the outcome. To efficiently conduct multiple sweeps of different parameters and determine their
effects, we have developed an in-vitro system to study the effects of LIFU on different types of cells grown in culture.
Presently, we are evaluating how LIFU affects the ionic flux that may underlie neuronal excitation and inhibition
observed in-vivo. The results of our in-vitro studies will provide a rationale for selection of optimal LIFU parameter to be
used in subsequent in-vivo applications. Thus, a prototype ultrasound cell assay system has been developed to conduct
these studies, and is described in this work.
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