Confocal laser scanning microscopy and electrochemical analysis were used to estimate the effectiveness of four blocking agents for biosensors preparation. The surface biomolecules layer was visualized. Among blocking agents under study, casein was chosen as the most suitable for electrochemical biosensors development.
Nanoconstructions of gold nanoparticles (NPs) obtained via pulsed laser ablation in liquid with DNA-aptamer specific to protein tumor marker were visualized on the surface of screen-printed electrode using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). AuNPs/aptamer nanoconstuctions distribution on the solid surface was studied. More uniform coverage of the carbon electrode surface with the nanoconstuctions was showed in comparison with DNA-aptamer alone on the golden electrode surface. Targeted binding of the tumor marker molecules with the AuNPs/DNA-aptamer nanoconstuctions was approved.
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