Dr. Andreas O. H. Gerstner Profile

Dr. Andreas O. H. Gerstner

at Rheinische Friedrich-Wilhelms-Univ. Bonn

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Publications (11)

Proceedings Article | 16 March 2020 Presentation + Paper

Spatio-spectral deep learning methods for in-vivo hyperspectral laryngeal cancer detection

Marcel Bengs, Stephan Westermann, Nils Gessert, Dennis Eggert, Andreas O. Gerstner, Nina Mueller, Christian Betz, Wiebke Laffers, Alexander Schlaefer

Proceedings Volume 11314, 113141L (2020) https://doi.org/10.1117/12.2549251

KEYWORDS: In vivo imaging, Tumors, Tissues, Cancer, Convolution, Neck, Head, Tissue optics, Image quality, Diagnostics

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Proceedings Article | 6 March 2020 Presentation

In vivo detection of laryngeal cancer by hyperspectral imaging combined with deep learning methods (Conference Presentation)

Dennis Eggert, Marcel Bengs, Stephan Westermann, Nils Gessert, Andreas O. Gerstner, Nina Müller, Alexander Schlaefer, Christian Betz, Wiebke Laffers

Proceedings Volume 11213, 112130L (2020) https://doi.org/10.1117/12.2557496

KEYWORDS: In vivo imaging, Hyperspectral imaging, Cancer, Tumors, Satellites, Surgery, Tissues, Convolutional neural networks, Convolution

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Proceedings Article | 29 March 2005 Paper

Immunophenotyping by slide-based cytometry and by flow cytometry are comparable

Andreas Gerstner, Wiebke Laffers, Anja Mittag, Ingo Daehnert, Domnik Lenz, Friedrich Bootz, Jozsef Bocsi, Attila Tarnok

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.588644

KEYWORDS: Luminescence, Flow cytometry, Blood, Glasses, Laser scanners, Statistical analysis, Signal detection, Cardiology, Laser systems engineering, Monoclonal antibodies

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Proceedings Article | 1 July 2004 Paper

Sequential photobleaching for increasing the measurable fluorochromes by laser scanning cytometry

Anja Mittag, Dominik Lenz, Jozsef Bocsi, Ulrich Sack, Andreas Gerstner, Attila Tarnok

Proceedings Volume 5322, (2004) https://doi.org/10.1117/12.528208

KEYWORDS: Luminescence, Optical filters, Blood, Laser scanners, Statistical analysis, Data acquisition, Glasses, Biological research, Fluorescence resonance energy transfer, Microscopes

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For immunophenotypic analysis more measurable parameters for the discrimination of leukocyte subsets are necessary. With a single scan six fluorochromes can be distinguished with the Laser Scanning Cytometer (LSC). Due to the number of PMTs the amount of simultaneously measurable fluorescences per scan is limited. Nevertheless, the amount of measurable colors can be improved to eight by appropriate change of the filter settings and two scans per specimen. Aim of this study was to use the special features of Slide based Cytometry (SBC) beyond filter change, remeasurement and merging to distinguish fluorochromes with similar emission spectra. The photosensitivity of fluorochromes that are excited and emit in a similar wavelength range may be very different. The number of measurable parameters per PMT was increased using photosensitivity of different fluorochromes as additional criteria. Peripheral blood leukocytes were stained with antibodies conjugated to the fluorochromes APC, APC-Cy5.5 and Alexa-Fluor 633 and mounted on conventional uncoated glass slides with Fluorescence mounting medium. Specimens were excited in the LSC with the HeNe (633nm) Laser and measured at different filter settings (670/20nm-filter for APC/ALEXA 633 and 710/20nm-filter for APC-Cy5.5). At this point, APC-Cy5.5 and APC/ALEXA633 were already distinguishable. In order to differentiate between APC and ALEXA633 photobleaching was performed by repeated excitation with the laser at 633nm. Control measurements proved that APC is much more sensitive against laser excitation, i.e. looses much more fluorescence intensity than ALEXA633. The separate measurements (before/after filter change and before/after bleaching) were merged into one file. The photostability of Alexa-Fluor 633 (1.02% bleach per scan) and APC (5.74% bleach per scan) are substantially different. Therefore, after bleaching and merging both fluorochromes can be distinguished and are regarded by the software as separate parameters. The fluorochromes APC/ALEXA633 and APC-Cy5.5 can be discriminated by changing the emission filters before bleach. By sequential photobleaching, change of filters and subsequent merging of the data the number of simultaneously measurable “colors” is substantially increased.

Proceedings Article | 22 July 2003 Paper

Clinical applications of slide-based cytometry (SBC)

Attila Taernok, Andreas Gerstner

Proceedings Volume 4958, (2003) https://doi.org/10.1117/12.476122

KEYWORDS: Luminescence, Tissues, Statistical analysis, Cell death, Tumors, Blood, Diagnostics, Biological research, Solids, Image analysis

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Proceedings Article | 19 June 2003 Paper

Use of DNA stains in immunophenotyping by slide-based cytometry

Andreas Gerstner, Wiebke Laffers, Friedrich Bootz, Attila Tarnok

Proceedings Volume 4962, (2003) https://doi.org/10.1117/12.477892

KEYWORDS: Luminescence, Blood, Surgery, Particles, Biological research, Sensors, Head, Neck, Microscopes, Optical filters

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Immunophenotyping of peripheral blood leukocytes (PBL) is a very well documented application of Slide Based Cytometry (SBC). As for any other assay it is of highest importance to ensure that all cells which are relevant for an analysis are recognized. Unlike assays for cultured cells which have homogenous morphology immunophenotyping of PBLs is performed on cells with heterogeneous size and shape. Therefore, triggering on parameters related to cell morphology might lead to an incomplete analysis of just a subset of cells especially in pathological conditions. Several dyes stain DNA specifically in a wide variety of emission spectra. Many of them show some influence of the chromatin condensation and organization on the staining intensity. DNA dyes therefore can be used to differentiate between cell types having the same ploidy. This can be exploited for immunophenotyping since some dyes therefore can partially replace antibody staining. The concept of using DNA dyes in the setting of immunostaining has the following advantages: (1) nuclear staining provides a stable and easy triggering signal that guarantees both, that neither cells are excluded nor that debris or polluting particles are included into the analysis; (2) some DNA dyes differentiate between mononuclear and polymorphonuclear cells. A disadvantage of DNA dyes is that mostly cells have to be permeabilized. Because of this only one set of immunophenotypic markers can be stained, cells are fixed and permeabilized, and then nuclei are stained with the appropriate DNA dye. In the study we demonstrate the use of the most commonly available DNA dyes (7-AAD, To-Pro, To-To, PI etc.) in their applicability in immunophenotyping. An overview of spectral properties, fluorescence spill-over and optimal combinations with surface antigen staining will be shown. As in general for SBC only very small sample volumes are needed. This allows to serially analyze PBL in clinical settings that up to now could not be studied in detail such as in the critical ill patient, during major surgery, and in new-borns and infants.

Proceedings Article | 19 June 2003 Paper

Six and more color immunophenotyping on the slide by laser scanning cytometry (LSC)

Dominik Lenz, Andreas Gerstner, Wiebke Laffers, Michae Steinbrecher, Friedrich Bootz, Attila Tarnok

Proceedings Volume 4962, (2003) https://doi.org/10.1117/12.477893

KEYWORDS: Optical filters, Luminescence, Bandpass filters, Blood, Laser scanners, Microscopes, Flow cytometry, Electronic filtering, Statistical analysis, Fluorescence correlation spectroscopy

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Proceedings Article | 4 June 2002 Paper

Multiparametric analysis of fine needle aspirate biopsies from parotid tumors by laser scanning cytometry (LSC)

Andreas Gerstner, Julia Machlitt, Anne-Kathrin Mueller, Attila Tarnok, Jens Oeken, Friedrich Bootz

Proceedings Volume 4625, (2002) https://doi.org/10.1117/12.469791

KEYWORDS: Tumors, Luminescence, Biological research, Biopsy, Neck, Nerve, Microscopes, Laser scanners, Photomicroscopy, Image analysis

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Proceedings Article | 28 May 2002 Paper

Novel approaches for immunophenotyping by laser scanning cytometry (LSC)

Andreas Gerstner, Wiebke Laffers, Friedrich Bootz, Dominik Lenz, Attila Tarnok

Proceedings Volume 4622, (2002) https://doi.org/10.1117/12.468349

KEYWORDS: Luminescence, Laser scanners, Microscopes, Blood, Flow cytometry, Optical filters, Statistical analysis, Laser therapeutics, Glasses, Photomicroscopy

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Proceedings Article | 28 May 2002 Paper

Multicolor immunophenotyping of tissue sections by laser scanning cytometry (LSC)

Attila Tarnok, Andreas Gerstner, Dominik Lenz, Pavel Osmancik, Peter Schneider, Christine Trumpfheller, Pal Racz, Klara Tenner-Racz

Proceedings Volume 4622, (2002) https://doi.org/10.1117/12.468348

KEYWORDS: Tissues, Luminescence, Lymphatic system, Data acquisition, Laser scanners, Argon ion lasers, Flow cytometry, Quantitative analysis, Microscopes, Laser tissue interaction

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Proceedings Article | 28 May 2002 Paper

Quantification of chemotaxis during pediatric cardiac surgery by flow and laser scanning cytometry

Attila Tarnok, Joerg Schmid, Pavel Osmancik, Dominik Lenz, Michal Pipek, Joerg Hambsch, Andreas Gerstner, Peter Schneider

Proceedings Volume 4622, (2002) https://doi.org/10.1117/12.468363

KEYWORDS: Surgery, Blood, Laser scanners, Optical filters, Flow cytometry, Luminescence, Laser therapeutics, Microscopes, Monoclonal antibodies, In vitro testing

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Showing 5 of 11 publications

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