Dr. Aneesh Alex Profile

Dr. Aneesh Alex

Optical Imaging Scientist at GlaxoSmithKline

SPIE Involvement:

Author

Publications (32)

Proceedings Article | 13 March 2024 Presentation

Presentation

Early selection of desired biopharmaceutical CHO cell lines using label-free multimodal optical microscopy and machine learning

Jindou Shi, Alexander Ho, Eric Chaney, Janet Sorrells, Kevin Tan, Aneesh Alex, Remben Talaban, Darold Spillman, Marina Marjanovic, Minh Doan, Steve Hood, Stephen Boppart

Proceedings Volume PC12846, PC1284603 (2024) https://doi.org/10.1117/12.3001298

KEYWORDS: Optical microscopy, Machine learning, Multiphoton fluorescence microscopy, Fluorescence lifetime imaging, Profiling, Ovary, Multiphoton microscopy, Multimodal imaging, Monte Carlo methods, Manufacturing

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Proceedings Article | 13 March 2024 Presentation

Presentation

Attention-based weakly-supervised deep learning for the identification and localization of drug fingerprints based on label-free hyperspectral CARS microscopy

Jindou Shi, Kajari Bera, Prabuddha Mukherjee, Aneesh Alex, Eric Chaney, Bradley Spencer-Dene, Jan Majer, Marina Marjanovic, Darold Spillman, Steve Hood, Stephen Boppart

Proceedings Volume PC12821, PC1282106 (2024) https://doi.org/10.1117/12.3001269

KEYWORDS: Microscopy, Deep learning, Biological samples, Nervous system, Complex systems, Vibration, Spatial learning, Profiling, Optical imaging, Nondestructive evaluation

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Proceedings Article | 13 March 2024 Presentation

Presentation

Academia to industry translation of SLAM technology

Aneesh Alex, Kayvan Tehrani, Mantas Zurauskas, Matthew Durack, Reid Groseclose, Steve Hood, Stephen Boppart

Proceedings Volume PC12821, PC128210B (2024) https://doi.org/10.1117/12.3003121

KEYWORDS: Industry, Microscopy, Microscopes, Image analysis, Biological research, Autofluorescence, Analytical research, 3D modeling, Visualization, Visual process modeling

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Proceedings Article | 13 March 2024 Presentation

Presentation

Predicting productivity in CHO cells based on co-occurrence of metabolism-related features in simultaneous label-free autofluorescence multiharmonic (SLAM) microscopy

Alexander Ho, Jindou Shi, Eric Chaney, Janet Sorrells, Kevin Tan, Aneesh Alex, Remben Talaban, Darold Spillman, Marina Marjanovic, Minh Doan, Steve Hood, Stephen Boppart

Proceedings Volume PC12854, PC1285407 (2024) https://doi.org/10.1117/12.3003094

KEYWORDS: Microscopy, Fluorescence lifetime imaging, Autofluorescence, Feature extraction, Support vector machines, Second harmonic generation, Proteins, Ovary, Multimodal imaging, Monte Carlo methods

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Proceedings Article | 17 March 2023 Presentation

Presentation

Visualizing and quantifying cisplatin-induced nephrotoxicity using nonlinear optical imaging

Aneesh Alex, Eric Chaney, Crysthiane Ishiy, Ciarán Fisher, Darold Spillman, Marina Marjanovic, Jan Roger, Reid Groseclose, Steve Hood, Stephen Boppart

Proceedings Volume 12357, 1235705 (2023) https://doi.org/10.1117/12.2649232

KEYWORDS: Visualization, Optical imaging, Tissue optics, Luminescence, Harmonic generation, Second-harmonic generation, Multiphoton microscopy, Microscopy, Medicine, Kidney

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Proceedings Article | 15 March 2023 Presentation

Presentation

Characterization of Chinese hamster ovary (CHO) cell lines by simultaneous label-free autofluorescence multi-harmonic (SLAM) microscopy for upstream bioprocessing (Conference Presentation)

Alexander Ho, Jindou Shi, Eric Chaney, Corey Snyder, Aneesh Alex, Remben Talaban, Janet Sorrells, Darold Spillman, Marina Marjanovic, Steve Hood, Stephen Boppart

Proceedings Volume PC12383, PC1238302 (2023) https://doi.org/10.1117/12.2646378

KEYWORDS: Microscopy, Ovary, Image processing, Luminescence, Image segmentation, Third-harmonic generation, Second-harmonic generation, Proteins, Multimodal imaging, Manufacturing

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Proceedings Article | 6 March 2023 Presentation

Presentation

Machine learning-assisted label-free multimodal optical bioimaging for biopharmaceutical CHO cell line characterization

Jindou Shi, Alexander Ho, Corey Snyder, Eric Chaney, Janet Sorrells, Aneesh Alex, Remben Talaban, Darold Spillman, Marina Marjanovic, Steve Hood, Stephen Boppart

Proceedings Volume PC12371, PC123710D (2023) https://doi.org/10.1117/12.2648499

KEYWORDS: Microscopy, Image segmentation, Fluorescence lifetime imaging, Feature extraction, Biomedical optics, Therapeutic antibodies, Proteins, Profiling, Ovary, Neural networks

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Proceedings Article | 7 March 2022 Presentation

Presentation

Differential uptake of antisense oligonucleotides in mouse hepatocytes and macrophages revealed by simultaneous 2P-fluorescence and coherent Raman imaging

Prabuddha Mukherjee, Edita Aksamitiene, Aneesh Alex, Jindou Shi, Kajari Bera, Chi Zhang, Darold Spillman, Marina Marjanovic, Michael Fazio, Punit Seth, Kendall Frazier, Steve Hood, Stephen Boppart

Proceedings Volume PC11938, PC119380D (2022) https://doi.org/10.1117/12.2608740

KEYWORDS: Coherence imaging, Luminescence, Raman spectroscopy, Liver, Receptors, Two photon imaging, Tissues, Multimodal imaging, Molecules, Modulation

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Proceedings Article | 7 March 2022 Presentation

Presentation

Label free and non-destructive imaging approach for cell line selection of genetically modified functional Chinese Hamster Ovary (CHO) Cells

Prabuddha Mukherjee, Edita Aksamitiene, Aneesh Alex, Jindou Shi, Eric Chaney, Remben Talaban, Maire Daly, Darold Spillman, Marina Marjanovic, Gary Finka, Steve Hood, Stephen Boppart

Proceedings Volume PC11964, PC119640C (2022) https://doi.org/10.1117/12.2608764

KEYWORDS: Ovary, Nondestructive evaluation, Fluorescence lifetime imaging, Optical imaging, Mode conditioning cables, Microscopy, Luminescence, Image analysis, Genetics, Cell biology

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Proceedings Article | 7 March 2022 Presentation

Presentation

Applying label free imaging to GSK drug discovery projects: current successes and future opportunities

Steve Hood, Aneesh Alex

Proceedings Volume PC11938, PC1193802 (2022) https://doi.org/10.1117/12.2606230

KEYWORDS: Drug development, Drug discovery, Tissue optics, Raman spectroscopy, Positron emission tomography, Optical tracking, Nonlinear optics, Network security, Monoclonal antibody therapy, Monoclonal antibodies

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Proceedings Article | 7 March 2022 Presentation

Presentation

Monitoring the delivery of lipid nanoparticle encapsulated self-amplifying mRNA vaccine using coherent Raman microscopy and multiphoton imaging

Kajari Bera, Prabuddha Mukherjee, Edita Aksamitiene, Aneesh Alex, Jindou Shi, Darold Spillman, Marina Marjanovic, Ahmed Shabana, Russell Johnson, Steve Hood, Stephen Boppart

Proceedings Volume PC11938, PC119380A (2022) https://doi.org/10.1117/12.2609087

KEYWORDS: Multiphoton microscopy, Microscopy, Nanoparticles, Raman spectroscopy, Green fluorescent protein, CARS tomography, Multiphoton fluorescence microscopy, Luminescence, Proteins, Multimodal imaging

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Proceedings Article | 7 March 2022 Presentation

Presentation

Nanodiamond based phantom for assessing multimodal fluorescence and multiharmonic imaging systems

Mantas Žurauskas, Aneesh Alex, Jaena Park, Steve Hood, Stephen Boppart

Proceedings Volume PC11951, PC1195103 (2022) https://doi.org/10.1117/12.2608258

KEYWORDS: Imaging systems, Luminescence, Microscopy, Control systems, Calibration, Microscopes, Harmonic generation, Scattering, Quality systems, Optical instrument design

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Proceedings Article | 2 March 2022 Presentation

Presentation

Label-free multimodal multiphoton imaging of Liver-on-a-Chip models

Aneesh Alex, Terrence Roh, BanuPriya Sridharan, Jindou Shi, Prabuddha Mukherjee, Eric Chaney, James Tunstead, Jan Majer, Kajari Bera, Darold Spillman, Marina Marjanovic, Jason Ekert, Steve Hood, Stephen Boppart

Proceedings Volume PC11972, PC1197202 (2022) https://doi.org/10.1117/12.2608861

KEYWORDS: Liver, Multiphoton microscopy, Multimodal imaging, 3D modeling, Multiphoton fluorescence microscopy, Microscopy, Optical imaging, Fluorescence lifetime imaging, Toxicology, Tissues

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Proceedings Article | 5 March 2021 Presentation

Presentation

Label-Free detection of antisense oligonucleotide uptake in Murine cells using hyperspectral CARS imaging

Prabuddha Mukherjee, Aneesh Alex, Edita Aksamitiene, Derek Shi, Darold Spillman, Marina Marjanovic, Michael Fazio, Punit Seth, Kendal Frazier, Steve Hood, Stephen Boppart

Proceedings Volume 11656, 116561F (2021) https://doi.org/10.1117/12.2578061

KEYWORDS: CARS tomography, Hyperspectral imaging, Microscopy, Raman spectroscopy, Proteins, Picosecond phenomena, Molecules, Luminescence, In vivo imaging, Imaging spectroscopy

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Proceedings Article | 5 March 2021 Presentation

Presentation

Enhancing liver on a chip Complex In Vitro Model studies using label-free optical imaging

Aneesh Alex, BanuPriya Sridharan, Edita Aksamitiene, Chi Zhang, Jindou Shi, Prabuddha Mukherjee, Mantas Zurauskas, Darold Spillman, Marina Marjanovic, Jason Ekert, Steve Hood, Stephen Boppart

Proceedings Volume 11624, 116240G (2021) https://doi.org/10.1117/12.2578531

KEYWORDS: Optical imaging, In vitro testing, Animal model studies, Multiphoton microscopy, Multiphoton fluorescence microscopy, Microscopy, 3D modeling, Tissues, Fluorescence lifetime imaging, Tumors

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Proceedings Article | 5 March 2021 Presentation

Presentation

Single-cell analysis pipeline for quantification of intracellular drug distribution by CARS/2PF/FLIM

Jindou Shi, Prabuddha Mukherjee, Aneesh Alex, Edita Aksamitiene, Darold Spillman, Marina Marjanovic, Minh Doan, Steve Hood, Stephen Boppart

Proceedings Volume 11649, 1164911 (2021) https://doi.org/10.1117/12.2577041

KEYWORDS: Image analysis, Fluorescence lifetime imaging, Optical imaging, Microscopy, Luminescence, CARS tomography

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Proceedings Article | 5 March 2021 Presentation

Presentation

Differential uptake of antisense oligonucleotides in mouse hepatocytes and macrophages revealed by simultaneous 2P-fluorescence and CARS

Prabuddha Mukherjee, Edita Aksamitiene, Aneesh Alex, Derek Shi, Darold Spillman, Marina Marjanovic, Michael Fazio, Punit Seth, Kendal Frazier, Steve Hood, Stephen Boppart

Proceedings Volume 11624, 116240E (2021) https://doi.org/10.1117/12.2578037

KEYWORDS: Two photon imaging, Receptors, Luminescence, Liver, Tissues, Multimodal imaging, Molecules, Modulation, Chemistry, CARS tomography

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Proceedings Article | 5 March 2021 Presentation

Presentation

FlimTK: a Python-based toolkit package for fluorescence lifetime imaging microscopy (FLIM) data analysis

Jindou Shi, Mantas Žurauskas, Prabuddha Mukherjee, Aneesh Alex, Darold Spillman, Marina Marjanovic, Steve Hood, Stephen Boppart

Proceedings Volume 11649, 1164907 (2021) https://doi.org/10.1117/12.2577939

KEYWORDS: Fluorescence lifetime imaging, Microscopy, Data analysis, Image analysis, Visualization, Visual analytics, Molecular biology, Luminescence, Image visualization, Graphics processing units

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Proceedings Article | 4 March 2019 Presentation

Presentation

Drug toxicity assessment using label-free multimodal nonlinear optical imaging (Conference Presentation)

Aneesh Alex, Jang Hyuk Lee, Jose Rico-Jimenez, Eric Chaney, Sunish Mohanan, Stephen Boppart, Steve Hood

Proceedings Volume 10859, 108590I (2019) https://doi.org/10.1117/12.2510032

KEYWORDS: Toxicity, Multimodal imaging, Optical imaging, Multiphoton microscopy, Multiphoton fluorescence microscopy, Microscopy, Fluorescence lifetime imaging, Toxicology, Drug development, Drug discovery

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Proceedings Article | 4 March 2019 Presentation

Presentation

Label-free multimodal multiphoton microscopy for monitoring of cell and tissue preservation and degradation in ex vivo whole organs (Conference Presentation)

Jang Hyuk Lee, Aneesh Alex, Jose Rico-Jimenez, Eric Chaney, Steve Hood, Stephen Boppart

Proceedings Volume 10881, 108810D (2019) https://doi.org/10.1117/12.2507482

KEYWORDS: Tissues, Multiphoton microscopy, Fluorescence lifetime imaging, Optical properties, Ultrafast imaging, Nonlinear optics, Light-matter interactions, Signal generators, Cell mechanics, Image processing

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Proceedings Article | 15 March 2018 Presentation

Presentation

Highly integrated label-free multiphoton nonlinear optical microspectroscopy imaging system for biomolecular imaging (Conference Presentation)

Jang Hyuk Lee, Jose Rico-Jimenez, Aneesh Alex, Eric Chaney, Ronit Barkalifa, Haohua Tu, Marina Marjanovic, Zane Arp, Stephen Boppart

Proceedings Volume 10505, 105050V (2018) https://doi.org/10.1117/12.2287194

KEYWORDS: Luminescence, Imaging systems, Imaging spectroscopy, Multiphoton fluorescence microscopy, Multiphoton microscopy, Microscopy, Spectroscopy, Optical coherence tomography, Fluorescence lifetime imaging, Harmonic generation

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Proceedings Article | 14 March 2018 Presentation

Presentation

Noninvasive monitoring of pharmacodynamics in the skin wound healing process using multimodal microscopy (Conference Presentation)

Jose Rico-Jimenez, Jang Hyuk Lee, Aneesh Alex, Eric Chaney, Ronit Barkalifa, Eric Olson, David Adams, Marina Marjanovic, Zane Arp, Stephen Boppart

Proceedings Volume 10475, 104750C (2018) https://doi.org/10.1117/12.2288621

KEYWORDS: Wound healing, Microscopy, Fluorescence lifetime imaging, Collagen, Skin, Optical coherence tomography, Angiography, Harmonic generation, Raman scattering, Multimodal imaging

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Proceedings Article | 14 March 2018 Presentation

Presentation

Detecting testicular toxicity using label-free multimodal nonlinear optical imaging (Conference Presentation)

Aneesh Alex, Jang Hyuk Lee, Jose Rico-Jimenez, Sunish Mohanan, Stephen Boppart, Zane Arp

Proceedings Volume 10486, 104860B (2018) https://doi.org/10.1117/12.2290646

KEYWORDS: Multiphoton microscopy, Multiphoton fluorescence microscopy, Microscopy, Toxicity, Optical imaging, Nonlinear optics, Safety, Fluorescence lifetime imaging, Tissue optics, Multimodal imaging

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Proceedings Article | 19 April 2017 Presentation

Presentation

Imaging bio-distribution of a topically applied dermatological cream on minipig skin using fluorescence lifetime imaging microscopy (Conference Presentation)

Aneesh Alex, Eric Chaney, Jennifer Criley, Darold Spillman, Phaedra Hutchison, Joanne Li, Marina Marjanovic, Steve Frey, Steven Cook, Stephen Boppart, Zane Arp

Proceedings Volume 10046, 100460E (2017) https://doi.org/10.1117/12.2253112

KEYWORDS: Optical tracking, Skin, Fluorescence lifetime imaging, Multiphoton fluorescence microscopy, Multiphoton microscopy, Microscopy, Optical imaging, Biopsy, Visualization, In vivo imaging

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Proceedings Article | 26 April 2016 Presentation

Presentation

An integrated optical coherence microscopy imaging and optical stimulation system for optogenetic pacing in Drosophila melanogaster (Conference Presentation)

Aneesh Alex, Airong Li, Jing Men, Jason Jerwick, Rudolph Tanzi, Chao Zhou

Proceedings Volume 9697, 96971A (2016) https://doi.org/10.1117/12.2214671

KEYWORDS: Imaging systems, Heart, Integrated optics, Optogenetics, Coherence imaging, Coherence (optics), Optical microscopy, Microscopy, Optical imaging, Tissues

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Proceedings Article | 26 April 2016 Presentation

Presentation

Optogenetic pacing in Drosophila melanogaster (Conference Presentation)

Aneesh Alex, Airong Li, Jing Men, Jason Jerwick, Rudolph Tanzi, Chao Zhou

Proceedings Volume 9690, 96902F (2016) https://doi.org/10.1117/12.2213877

KEYWORDS: Heart, Optogenetics, Tissues, Tissue optics, In vivo imaging, Animal model studies, Imaging systems, Gold, Standards development, Optical coherence tomography

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Proceedings Article | 18 June 2013 Paper

Paper

Advanced integrated spectrometer designs for miniaturized optical coherence tomography systems

B. Akca, B. Považay, L. Chang, A. Alex, K. Wörhoff, R. de Ridder, W. Drexler, M. Pollnau

Proceedings Volume 8802, 880202 (2013) https://doi.org/10.1117/12.2032504

KEYWORDS: Optical coherence tomography, Spectroscopy, Polarization, Imaging systems, System integration, Waveguides, Refractive index, In vivo imaging, Spectrometer engineering, Stars

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Proceedings Article | 3 February 2012 Paper

Paper

Three-dimensional calibration targets for optical coherence tomography

Michelle Gabriele Sandrian, Pete Tomlins, Peter Woolliams, Janarthanan Rasakanthan, Graham Lee, Anna Yang, Boris Považay, Aneesh Alex, Kate Sugden, Wolfgang Drexler

Proceedings Volume 8229, 822914 (2012) https://doi.org/10.1117/12.907748

KEYWORDS: Optical coherence tomography, Calibration, Nanoparticles, Point spread functions, 3D acquisition, Femtosecond phenomena, Particles, 3D metrology, Iron, Oxides

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Proceedings Article | 16 February 2011 Paper

Paper

Multimodal optical coherence/photoacoustic tomography of skin

Aneesh Alex, Edward Zhang, Boris Považay, Jan Laufer, Bernd Hofer, Carl Glittenberg, Boris Hermann, Paul Beard, Wolfgang Drexler

Proceedings Volume 7889, 788916 (2011) https://doi.org/10.1117/12.875499

KEYWORDS: Optical coherence tomography, Skin, Acquisition tracking and pointing, Imaging systems, Sensors, Tissue optics, In vivo imaging, Tissues, Tomography, Multimodal imaging

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SPIE Journal Paper | 1 March 2010 Open Access

Open Access

Multispectral in vivo three-dimensional optical coherence tomography of human skin

Alex Aneesh, Boris Považay, Bernd Hofer, Sergei Popov, Carl Glittenberg, Susanne Binder, Wolfgang Drexler

JBO, Vol. 15, Issue 02, 026025, (March 2010) https://doi.org/10.1117/12.10.1117/1.3400665

KEYWORDS: Optical coherence tomography, Skin, Tissues, Scattering, In vivo imaging, Image resolution, 3D image processing, Light scattering, Biomedical optics, Visualization

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Proceedings Article | 24 February 2010 Paper

Paper

Multimodal simultaneous photoacoustic tomography, optical resolution microscopy, and OCT system

Edward Zhang, Jan Laufer, Boris Považay, Aneesh Alex, Bernd Hofer, Wolfgang Drexler, Paul Beard

Proceedings Volume 7564, 75640U (2010) https://doi.org/10.1117/12.841615

KEYWORDS: Acquisition tracking and pointing, Optical coherence tomography, Sensors, Tissues, Ultrasonography, Acoustics, Signal detection, Photoacoustic spectroscopy, Spatial resolution, Photoacoustic tomography

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Proceedings Article | 20 February 2010 Paper

Paper

Optimizing penetration depth, contrast, and resolution in 3D dermatologic OCT

Alex Aneesh, Boris Považay, Bernd Hofer, Edward Zhang, Catherine Kendall, Jan Laufer, Sergei Popov, Carl Glittenberg, Susanne Binder, Nicholas Stone, Paul Beard, Wolfgang Drexler

Proceedings Volume 7554, 75540V (2010) https://doi.org/10.1117/12.843243

KEYWORDS: Optical coherence tomography, Skin, 3D image processing, Imaging systems, Image resolution, Tissues, Tumors, In vivo imaging, Tissue optics, Signal to noise ratio

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Showing 5 of 32 publications

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