Dr. Ashley M. Laughney Profile

Dr. Ashley M. Laughney

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Publications (14)

Proceedings Article | 4 March 2015 Paper

Multispectral reflectance enhancement for breast cancer visualization in the operating room

Gaspar Fernandez-Barreras, Eusebio Real, Ashley Laughney, Venkataramanan Krishnaswamy, Keith Paulsen, José López-Higuera, Brian Pogue, Olga Conde

Proceedings Volume 9311, 931106 (2015) https://doi.org/10.1117/12.2078774

KEYWORDS: Tissues, Reflectivity, Scattering, Tumors, Breast, Visualization, Surgery, Image enhancement, Absorption, Breast cancer

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SPIE Journal Paper | 22 March 2013 Open Access

System analysis of spatial frequency domain imaging for quantitative mapping of surgically resected breast tissues

Ashley Laughney, Venkataramanan Krishnaswamy, Tyler Rice, David Cuccia, Richard Barth, Bruce Tromberg, Keith Paulsen, Brian Pogue, Wendy Wells

JBO, Vol. 18, Issue 03, 036012, (March 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.3.036012

KEYWORDS: Imaging systems, Absorption, Breast, Diffusion, Modulation, Light scattering, Spatial frequencies, Surgery, Tissues, Scattering

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Proceedings Article | 21 February 2013 Paper

Fractal analysis of scatter imaging signatures to distinguish breast pathologies

Alma Eguizabal, Ashley Laughney, Venkataramanan Krishnaswamy, Wendy Wells, Keith Paulsen, Brian Pogue, José López-Higuera, Olga Conde

Proceedings Volume 8592, 85920Y (2013) https://doi.org/10.1117/12.2003830

KEYWORDS: Fractal analysis, Tissues, Scattering, Tumors, Principal component analysis, Breast, Reflectivity, Tumor growth modeling, Pathology, Statistical modeling

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Proceedings Article | 21 February 2013 Paper

Linear classifier and textural analysis of optical scattering images for tumor classification during breast cancer extraction

Alma Eguizabal, Ashley Laughney, Pilar Beatriz Garcia Allende, Venkataramanan Krishnaswamy, Wendy Wells, Keith Paulsen, Brian Pogue, José López-Higuera, Olga Conde

Proceedings Volume 8592, 85920E (2013) https://doi.org/10.1117/12.2003814

KEYWORDS: Tissues, Scattering, Tissue optics, Diagnostics, Breast cancer, Light scattering, Statistical analysis, Tumors, Surgery, Mie scattering

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Proceedings Article | 2 February 2012 Paper

Blind breast tissue diagnosis using independent component analysis of localized backscattering response

Alma Eguizabal, Ashley Laughney, Pilar Beatriz García Allende, Venkataramanan Krishnaswamy, Wendy Wells, Keith Paulsen, Brian Pogue, Jose Lopez-Higuera, Olga Conde

Proceedings Volume 8230, 823014 (2012) https://doi.org/10.1117/12.909326

KEYWORDS: Independent component analysis, Scattering, Tissues, Breast, Mie scattering, Tissue optics, Reflectivity, Diagnostics, Surgery, Pathology

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Proceedings Article | 2 February 2012 Paper

Enhanced tumor contrast during breast lumpectomy provided by independent component analysis of localized reflectance measures

Alma Eguizabal, Ashley Laughney, Pilar Beatriz Garcia Allende, Venkataramanan Krishnaswamy, Wendy Wells, Keith Paulsen, Brian Pogue, Jose Lopez-Higuera, Olga Conde

Proceedings Volume 8230, 823010 (2012) https://doi.org/10.1117/12.909348

KEYWORDS: Independent component analysis, Tissues, Scattering, Reflectivity, Breast, Principal component analysis, Tumors, Model-based design, Statistical analysis, Diagnostics

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Proceedings Article | 12 February 2011 Paper

A dark-field scanning spectroscopy platform for localized scatter and fluorescence imaging of tissue

Venkataramanan Krishnaswamy, Ashley Laughney, Keith Paulsen, Brian Pogue

Proceedings Volume 7907, 79070J (2011) https://doi.org/10.1117/12.875462

KEYWORDS: Spectroscopy, Luminescence, Fluorescence spectroscopy, Tissue optics, Imaging spectroscopy, Imaging systems, Tissues, Molecular spectroscopy, Absorption, Spectroscopes

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Proceedings Article | 12 February 2011 Paper

Optical assessment of pathology in surgically resected tissues

Ashley Laughney, Venkataramanan Krishnaswamy, Wendy Wells, Olga Conde, Keith Paulsen, Brian Pogue

Proceedings Volume 7907, 79070P (2011) https://doi.org/10.1117/12.874059

KEYWORDS: Tissues, Scattering, Pathology, Spectroscopy, Modulation, Imaging spectroscopy, Tissue optics, Absorption, Breast, Tumors

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Proceedings Article | 11 February 2011 Paper

Assessment of biophysical tumor response to PDT in pancreatic cancer using localized reflectance spectroscopy

Martin Isabelle, William Klubben, Ting He, Ashley Laughney, Adam Glaser, Venkataramanan Krishnaswamy, P. Jack Hoopes, Tayyaba Hasan, Brian Pogue

Proceedings Volume 7886, 78860S (2011) https://doi.org/10.1117/12.876147

KEYWORDS: Tissues, Tumors, Photodynamic therapy, Tissue optics, Reflectivity, Inflammation, Imaging systems, Chromophores, Absorbance, Reflectance spectroscopy

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Biophysical changes such as inflammation and necrosis occur immediately following PDT and may be used to assess the treatment response to PDT treatment in-vivo. This study uses localized reflectance measurements to quantify the scatter changes in tumor tissue occurring in response to verteporfin-based PDT treatment in xenograft pancreas tumors. Nude mice were implanted with subcutaneous AsPC-1 pancreatic tumors cells in matrigel, and allowed to establish solid tumors near 100mm3 volume. The mice were sensitized with 1mg/kg of the active component of verteporfin (benzoporphryin derivative, BPD), one hour before light delivery. The optical irradiation was performed using a 1 cm cylindrical interstitial diffusing tip fiber with 20J of red light (690nm). Tumor tissue was excised progressively and imaged, from 1 day to 4 weeks, after PDT treatment. The tissue sections were stained and analyzed by an expert veterinary pathologist, who provided information on tissue regions of interest. This information was correlated with variations in scattering and absorption parameters elucidated from the spectral images and the degree of necrosis and inflammation involvement was identified. Areas of necrosis and dead cells exhibited the lowest average scatter irradiance signature (3.78 and 4.07 respectively) compared to areas of viable pancreatic tumor cells and areas of inflammation (5.81 and 7.19 respectively). Bilirubin absorbance parameters also showed a lower absorbance value in necrotic tissue and areas of dead cells (0.05 and 0.1 respectively) compared to tissue areas for viable pancreatic tumor cells and areas of inflammation (0.28 and 0.35). These results demonstrate that localized reflectance spectroscopy is an imaging modality that can be used to identify tissue features associated with PDT treatment (e.g. necrosis and inflammation) that can be correlated with histopathologically-reviewed H&E stained slides. Further study of this technique may provide means for automated discrimination of tissue features based on scatter and absorbance maps elucidated from reflectance spectral datasets and provide a valuable tool for treatment response monitoring during PDT and enabling more effective treatment planning. These results are relevant to verteporfin-based PDT trial for treatment pancreatic cancer in non-surgical candidate cases (VERTPAC-1 University College London, PI Pereira), where individualized assessment of damage and response could be beneficial, if this study is proven to be a well-controlled imaging tool.

SPIE Journal Paper | 1 November 2010 Open Access

Automated classification of breast pathology using local measures of broadband reflectance

Ashley Laughney, Venkataramanan Krishnaswamy, Pilar Beatriz García-Allende, Olga Conde, Wendy Wells, Keith Paulsen, Brian Pogue

JBO, Vol. 15, Issue 06, 066019, (November 2010) https://doi.org/10.1117/12.10.1117/1.3516594

KEYWORDS: Error analysis, Light scattering, Diagnostics, Surgery, Reflectivity, Tissue optics, Breast, Scattering, Pathology, Tissues

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Proceedings Article | 24 February 2009 Paper

A multi-spectral dark-field reflectance microscope for tumor margin delineation during breast lumpectomy

Venkataramanan Krishnaswamy, Wendy Wells, Ashley Laughney, Kimberley Samkoe, Brian Pogue

Proceedings Volume 7187, 718710 (2009) https://doi.org/10.1117/12.809193

KEYWORDS: Light scattering, Imaging systems, Scattering, Tissue optics, Reflectivity, Mirrors, Tissues, Zemax, Tumors, Breast

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Proceedings Article | 12 February 2009 Paper

Pressure-enhanced near-infrared breast imaging: toward cancer patient imaging

Shudong Jiang, Brian Pogue, Ashley Laughney, Keith Paulsen

Proceedings Volume 7174, 71740S (2009) https://doi.org/10.1117/12.808071

KEYWORDS: Blood, Breast, Imaging systems, Data acquisition, Chromophores, Breast cancer, Tissues, Breast imaging, Near infrared spectroscopy, Cancer

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Proceedings Article | 12 February 2009 Paper

Biophysical interpretation and ex-vivo characterization of scattered light from tumor-associated breast stroma

Ashley Laughney, Venkat Krishnaswamy, Mary Schwab, Wendy Wells, Keith Paulsen, Brian Pogue

Proceedings Volume 7174, 71741Y (2009) https://doi.org/10.1117/12.808323

KEYWORDS: Tissues, Collagen, Breast, Phase contrast, Tissue optics, Tumors, Reflectance spectroscopy, Transmission electron microscopy, Diagnostics, Microscopy

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Proceedings Article | 20 February 2008 Paper

System design for spectrally encoded video-rate near infrared tomography during magnetic resonance imaging of the breast

Brian Pogue, Zhiqiu Li, Colin Carpenter, Ashley Laughney, Venkataramanan Krishnaswamy, Scott Davis, Shudong Jiang, Keith Paulsen

Proceedings Volume 6850, 68500H (2008) https://doi.org/10.1117/12.778326

KEYWORDS: Breast, Magnetic resonance imaging, Spectroscopy, Sensors, Tissues, Tomography, Breast imaging, Near infrared, Light, Imaging systems

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Showing 5 of 14 publications

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