In this paper we describe a facile method for synthesizing gold triangular nanoframe from edge gold-coated silver
nanoprism [Ag@(Au nanoframe)] with controlled ridge thickness and interior hollow diameter via chemical etching of
silver from the gold coated Ag nanoprism with Hydrogen peroxide. Our process involves two major steps: (1) Formation
of Ag@(Au nanoframe) by deposition of gold on the edges of Ag nanoprisms in room temperature (2) Etching of Ag
atoms from the gold fame via H2O2 oxidation. The optical properties of the resulting nanoframe can be easily tailored in
a broad range by controlling the ridge thickness or hole diameter by amount of H2O2 added into the reaction. Due to the
changes to the optical spectra during etching, the nanoframe formation can be employed to detect H2O2 in a broad range
of concentration down to 1 μM.
Heterodimerization between designed helix-loop-helix polypeptides was utilized in order to assemble gold nanoparticles
on planar substrates. The peptides were designed to fold into four-helix bundles upon dimerization. A Cys-residue in the
loop region was used to immobilize one of the complementary peptides on a maleimide containing SAM on planar gold
substrates whereas the second peptide was immobilized directly on gold nanoparticles. Introducing the peptide
decorated particles over a peptide functionalized surface resulted in particle assembly. Further, citrate stabilized
particles were assembled on amino-silane modified glass and silicon substrates. By subsequently introducing peptides
and gold nanoparticles, particle-peptide hybrid multi layers could be formed.
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