By Mueller-matrix mapping of changes in the distribution of the magnitude of matrix invariants characterizing the degree of crystallization of histological sections of the brain, liver and kidney, the forensic medical criteria were determined and the interval for determining the damage duration of 24 hours with an accuracy of 45min. - 50 min. was established. For azimuthally invariant polarization mapping of changes in the distributions of Mueller-matrix invariants characterizing the optical activity of molecular complexes of histological sections of the brain, liver, and kidney, forensic medical criteria were developed for the first time and accuracy was improved to 35 min. - 40 min. in the interval for determining the damage duration period of 72 hours. For highly accurate objective determination of the damage duration in a long time interval (1 hour - 120 hours), a polarization tomography method has been developed (reproducing the distributions of birefringence of fibrillar networks of histological sections of internal organs), which provides an accuracy of 25 minutes. (1 hour - 24 hours) to 45 minutes (24 hours - 120 hours).
For a high-precision objective histological determination of the prescription of damage to internal organs over a long period of time, a systematic approach was used based on digital azimuthally invariant polarizing, Mueller-matrix and tomographic methods for studying temporary changes in the molecular and polycrystalline structure of brain, liver and kidney samples in the post-mortal period. It was revealed that a linear change in the magnitude of statistical moments of the 1st - 4th orders characterizing the distribution of data of digital azimuthally invariant polarizing, Mueller-matrix and tomographic methods is interconnected with the duration of damage to internal organs. On this basis, a new algorithm for digital histological determination of the prescription of the occurrence of damage is proposed. To determine the extent of damage, the method of azimuthally invariant polarization microscopy with different magnification of the image of histological sections of tissues of internal organs was used, which provided diagnostic relationships between changes in the magnitude of statistical moments of the 1st to 4th orders, which characterize the azimuth and elliptic polarization maps of digital microscopic images and time intervals of damage duration.
The results of autofluorescence polarimetry of microscopic images of histological sections of tissues of rat internal organs are presented. The coordinate distributions of the magnitude of the 3rd and 4th parameters of the Stokes vector are investigated. In the framework of the statistical approach, the values and ranges of changes of the 1st - 4th statistical moments characterizing such parameters of microscopic images of healthy and sick (diabetes) rats were determined. The balanced accuracy of differentiation of this pathology was established by the method of autofluorescence polarimetry.
The results of the study of the relationships between 3D divisions of the optical anisotropy parameters of polycrystalline networks of films of biological fluids of various biochemical composition and layer-by-layer phase cross sections of volume distributions of the magnitude and phase parameters of the "two-point" Stokes vector of a microscopic image are presented. In the framework of the statistical approach using scale-selective wavelet analysis, the values and ranges of statistical 1st-4th order changes are defined, which characterize: • distribution of the values of the modulus and phase of the parameters of the Stokes vector in various phase sections of the object field; • a set of values of the amplitudes of the wavelet coefficients for various scales of the geometric dimensions of the module maps and the phase of the degree of correlation of the parameters of the Stokes vector (DCS).
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