We have developed a solid-state true-color charge-coupled (CCD) imager for nanosecond time-gated fluorescence
imaging. Unlike intensifier-based imagers, the Peltier-cooled CCD imager is directly gated and hence has very low
noise. The excitation source used with the imager is a compact diode-pumped nanosecond pulsed laser giving outputs at
532nm and 355nm with a variable repetition rate up to more than 10kHz. The operation of the new imager has been
demonstrated using fluorescent samples that differ both in emission spectrum and in fluorescence decay time. Potential
applications of the new technology are discussed including multiplexed detection of labels based on the combination of
emission spectrum and fluorescence lifetime.
Quantum dots are now recognised as valuable luminescent labels. Amongst their desirable characteristics is a relatively
long luminescence decay time that can allow selective detection in the presence of shorter lived background
fluorescence. Here we demonstrate that nanosecond time-resolved imaging of quantum dot samples is easily accomplished with a directly-gated cooled CCD system that does not require an image intensifier stage to achieve nanosecond time resolution. The approach is likely to find application both to imaging of quantum dots in the presence
of autofluorescence from tissues and also for time-resolved measurements of resonance energy transfer from quantum
dot donors to conventional acceptor labels.
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