The usage of UV-LEDs is growing for a variety of applications such as the disinfection of water, the sterilization of medical equipment and the industrial UV curing. It is important that the wavelength and the intensity of radiation needed for the specific application is reliably provided and covers the whole target area evenly distributed.
The individual high precision power supply and control of UV-LEDs is the main application of the eyIC4UV-01 chip. The eyIC4UV-01 is an integrated circuit that can measure the light intensity, temperature, current and forward voltage of a connected UV-LED.
Characterising the amount and the purity of nucleic acid is an important step in state of the art polymerase chain reaction (PCR). In most cases, the analysis is done by stand-alone equipment. For the measurement, a small amount out of the PCR-process has to be removed. Furthermore, the evaluation of the measured spectra occurs only at three wavelengths (230 nm, 260 nm, 280 nm). Therefore, it should be possible to monitor the PCR-process in situ. We demonstrate an illumination unit with three UV-LEDs (245 nm, 265 nm and 280 nm). Every LED is collimated by two lenses. Two longwave-pass filters merge the optical axes of the different wavelength. Lenses and filters are commercial available. The illumination unit is available with and without fiber coupling. The optical behavior of the illumination unit will be shown and discussed. Further, we investigate the observed peak position of the supporting points in dependence of the impurity concentration of an example solution.
Fluorescence lifetime determination is widely utilized for bioscience research and analysis. The fluorescence stimulation in conventional systems is usually done with expensive picosecond laser systems. We present a cost-effective 370 nm LED based excitation module and a detection unit based on a Silicon Photomultiplier (SiPM). The functionality of the excitation module as well as the detection module is demonstrated with the fluorescence dye ATTO 390.
For a fast analysis of the fluorescence signal detected by the SiPM, we developed an ASIC for fluorescence histogram recording. The ASIC determines the time between excitation pulse and incoming fluorescence photon with an accuracy of about 80 ps. The ASIC blind time after the excitation pulse is configurable. The determined time is saved in bins. The width of the bins is programmable. Output of the ASIC is a histogram with the counted amount of photons at the different times after excitation. This histogram equals the fluorescence response of the dye. The fluorescence lifetime can be calculated out of this histogram.
Fluorescence lifetime determination is widely utilized for bioscience research and analysis. The fluorescence stimulation in conventional systems is usually done with expensive picosecond laser systems. We present a cost-effective 370 nm LED based excitation module with a pulse FWHM of 1 ns and a beam diameter of 4 mm. The functionality of the excitation module was demonstrated with the fluorescence dye ATTO 390 with a fluorescence lifetime of 5 ns. The width of 8 mm of the excitation module enables the parallel measurement of adjacent sample chambers of a well plate. Further, a silicon UV-photodiode is designated to monitor the output power of the LED. For a fast analysis of the fluorescence signal, we developed an ASIC for fluorescence histogram recording. The ASIC determines the time between excitation pulse and incoming fluorescence photon with an accuracy of about 80 ps. The ASIC blind time after the excitation pulse is configurable. The determined time is saved in bins. The width of the bins is programmable. For fluorescence light detection a silicon photomultiplier (SiPM) is used. Output of the ASIC is a histogram with the counted amount of photons at the different times after excitation. This histogram equals the fluorescence response of the dye. The fluorescence lifetime can be calculated out of this histogram.
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