Dr. Daniel L. Farkas Profile

Dr. Daniel L. Farkas

Chairman at Univ of Southern California

SPIE Involvement:

Track Chair | Editor | Author

Publications (59)

Proceedings Article | 14 May 2019 Presentation

Multimode optical imaging for identification of fish fillet substitution and mislabeling (Conference Presentation)

Fartash Vasefi, Rachel Isaacs , Stas Sokolov, Li Kang, Rosalee Hellberg , Daniel Farkas , Jianwei Qin , Diane Chan , Moon Kim

Proceedings Volume 11016, 1101606 (2019) https://doi.org/10.1117/12.2523224

KEYWORDS: Optical imaging, Imaging spectroscopy, Imaging systems, Reflectivity, Image processing, Visible radiation, Near infrared, Short wave infrared radiation, Luminescence, Raman spectroscopy

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Proceedings Article | 4 March 2019 Presentation

Ultra-resolution spectral confocal add-on systems for light microscopes (Conference Presentation)

Fartash Vasefi, Kevin Burton, Nicholas Booth, Daniel Farkas

Proceedings Volume 10881, 1088118 (2019) https://doi.org/10.1117/12.2516234

KEYWORDS: Confocal microscopy, Microscopes, Laser scanners, Image enhancement, Spectral resolution, Laser tissue interaction, Molecular biology, Pathology, Fluorescence resonance energy transfer, Resonance enhancement

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Proceedings Article | 11 June 2018 Presentation

Mie scattering characterization by ultra-spectral illumination using micro-sized tunable liquid crystal optical filters (Conference Presentation)

Fartash Vasefi, Russell Barbour, Caleb Stoltzfus, Zeb Barber, Daniel Farkas

Proceedings Volume 10497, 104971D (2018) https://doi.org/10.1117/12.2292620

KEYWORDS: Mie scattering, Liquid crystals, Biological research, Scattering, Optical filters, Particles, Tissues, Optical microcavities, Confocal microscopy, Light scattering

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Proceedings Article | 15 May 2018 Presentation

Multimode optical imaging for food quality and safety applications (Conference Presentation)

Fartash Vasefi, Danielle Rosen, Nicholas Booth, Hesam Hafizi, Li Kang, Rosalee Hellberg, Daniel Farkas

Proceedings Volume 10665, 1066507 (2018) https://doi.org/10.1117/12.2317850

KEYWORDS: Safety, Imaging systems, Optical imaging, Reflectivity, Luminescence, Light emitting diodes, Hyperspectral imaging, Image acquisition, Light sources, LED lighting

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Proceedings Article | 24 April 2017 Presentation

In vivo features of melanocytic lesions: multimode hyperspectral dermoscopy, reflectance confocal microscopy, and histopathologic correlates (Conference Presentation)

Fartash Vasefi, Nicholas MacKinnon, Manu Jain, Miguel Cordova, Kivanc Kose, Milind Rajadhyaksha, Allan Halpern, Daniel Farkas

Proceedings Volume 10068, 100680G (2017) https://doi.org/10.1117/12.2261499

KEYWORDS: Skin, In vivo imaging, Melanoma, Reflectivity, Confocal microscopy, Diagnostics, Standards development, Imaging systems, 3D image processing, Cancer

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Proceedings Article | 24 April 2017 Presentation

Improved heuristics for early melanoma detection using multimode hyperspectral dermoscopy (Conference Presentation)

Fartash Vasefi, Nicholas MacKinnon, Nicholas Booth, Daniel Farkas

Proceedings Volume 10068, 100680H (2017) https://doi.org/10.1117/12.2261501

KEYWORDS: Melanoma, Skin, In vivo imaging, Polarization, Luminescence, Hyperspectral imaging, Collagen, Imaging systems, Absorption, Feature extraction

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SPIE Journal Paper | 26 December 2016 Open Access

Review of the potential of optical technologies for cancer diagnosis in neurosurgery: a step toward intraoperative neurophotonics

Fartash Vasefi, Nicholas MacKinnon, Daniel Farkas, Babak Kateb

NPh, Vol. 4, Issue 01, 011010, (December 2016) https://doi.org/10.1117/12.10.1117/1.NPh.4.1.011010

KEYWORDS: Tumors, Tissues, Brain, Tissue optics, Luminescence, Raman spectroscopy, Cancer, Neurophotonics, Neuroimaging, Optical coherence tomography

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SPIE Journal Paper | 10 November 2016 Open Access

Separating melanin from hemodynamics in nevi using multimode hyperspectral dermoscopy and spatial frequency domain spectroscopy

Fartash Vasefi, Nicholas MacKinnon, Rolf Saager, Kristen Kelly, Tyler Maly, Nicholas Booth, Anthony Durkin, Daniel Farkas

JBO, Vol. 21, Issue 11, 114001, (November 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.11.114001

KEYWORDS: Skin, Absorption, Hemodynamics, Tissue optics, Ultrasonography, Spectroscopy, Melanoma, Signal attenuation, Polarization, Imaging systems

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Proceedings Article | 6 April 2016 Presentation + Paper

Melanoma detection using smartphone and multimode hyperspectral imaging

Nicholas MacKinnon, Fartash Vasefi, Nicholas Booth, Daniel Farkas

Proceedings Volume 9711, 971117 (2016) https://doi.org/10.1117/12.2222415

KEYWORDS: Hyperspectral imaging, Imaging systems, RGB color model, Skin, Image quality, Melanoma, Dermatology, Polarization, Medicine, Tissues

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Proceedings Article | 6 April 2016 Paper

Multimode optical dermoscopy (SkinSpect) analysis for skin with melanocytic nevus

Fartash Vasefi, Nicholas MacKinnon, Rolf Saager, Kristen Kelly, Tyler Maly, Robert Chave, Nicholas Booth, Anthony Durkin, Daniel Farkas

Proceedings Volume 9711, 971110 (2016) https://doi.org/10.1117/12.2214288

KEYWORDS: Skin, In vivo imaging, Tissue optics, Biological research, Tissues, Statistical analysis, Modulation, Spectroscopes, Melanoma, Hyperspectral imaging

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Proceedings Article | 25 March 2015 Paper

Quantifying the optical properties of turbid media using polarization sensitive hyperspectral imaging (SkinSpect): two-layer optical phantom studies

Fartash Vasefi, Nicholas MacKinnon, Rolf Saager, Anthony Durkin, Robert Chave, Daniel Farkas

Proceedings Volume 9328, 93280A (2015) https://doi.org/10.1117/12.2186031

KEYWORDS: Polarization, Absorption, Optical properties, Melanoma, Signal attenuation, Skin, Hyperspectral imaging, Imaging systems, Tissue optics, Scattering

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SPIE Journal Paper | 21 March 2014 Open Access

High-speed multispectral confocal biomedical imaging

Gary Carver, Sarah Locknar, William Morrison, V. Krishnan Ramanujan, Daniel Farkas

JBO, Vol. 19, Issue 03, 036016, (March 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.3.036016

KEYWORDS: Confocal microscopy, Tissues, Multispectral imaging, Biomedical optics, Luminescence, Spectroscopy, Tumors, Endoscopy, Sensors, Microscopy

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Proceedings Article | 4 March 2014 Paper

Multispectral imaging for diagnosis and treatment

Gary Carver, Sarah Locknar, William Morrison, Daniel Farkas

Proceedings Volume 8947, 89470L (2014) https://doi.org/10.1117/12.2039980

KEYWORDS: Tissues, Confocal microscopy, Tissue optics, Multispectral imaging, Spectroscopy, Luminescence, Spatial resolution, Endoscopy, Software development, Laser tissue interaction

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Proceedings Article | 4 March 2014 Paper

Toward in vivo diagnosis of skin cancer using multimode imaging dermoscopy: (II) molecular mapping of highly pigmented lesions

Fartash Vasefi, Nicholas MacKinnon, Daniel Farkas

Proceedings Volume 8947, 89470J (2014) https://doi.org/10.1117/12.2042182

KEYWORDS: Skin, Signal attenuation, Absorption, Polarization, Absorbance, Chromophores, Tissue optics, Blood, Hyperspectral imaging, RGB color model

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Proceedings Article | 4 March 2014 Paper

Toward in vivo diagnosis of skin cancer using multimode imaging dermoscopy: (I) clinical system development and validation

Nicholas MacKinnon, Fartash Vasefi, Daniel Farkas

Proceedings Volume 8947, 89470I (2014) https://doi.org/10.1117/12.2041818

KEYWORDS: Skin, Polarization, Prototyping, Melanoma, Luminescence, Tissues, Imaging systems, Skin cancer, Hyperspectral imaging, Cameras

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Proceedings Article | 26 February 2013 Paper

Quantifying the optical properties and chromophore concentrations of turbid media using polarization sensitive hyperspectral imaging: optical phantom studies

Fartash Vasefi, Rolf Saager, Anthony Durkin, Nicholas MacKinnon, Eugene Gussakovsky, Robert Chave, Daniel Farkas

Proceedings Volume 8587, 85870Z (2013) https://doi.org/10.1117/12.2005371

KEYWORDS: Skin, Absorption, Optical properties, Scattering, Tissue optics, Polarization, Hyperspectral imaging, Imaging systems, Near infrared, Titanium dioxide

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Proceedings Article | 22 February 2013 Paper

In vivo skin chromophore mapping using a multimode imaging dermoscope (SkinSpect™)

Nicholas MacKinnon, Fartash Vasefi, Eugene Gussakovsky, Gregory Bearman, Robert Chave, Daniel Farkas

Proceedings Volume 8587, 85870U (2013) https://doi.org/10.1117/12.2005587

KEYWORDS: Skin, RGB color model, Data modeling, Polarization, Tissues, Chromophores, Reflectivity, Melanoma, Tissue optics, Luminescence

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Proceedings Article | 22 February 2013 Paper

High-speed multispectral confocal imaging

Gary Carver, Sarah Locknar, William Morrison, Daniel Farkas

Proceedings Volume 8587, 858715 (2013) https://doi.org/10.1117/12.2001883

KEYWORDS: Confocal microscopy, Tissues, Multispectral imaging, Spectroscopy, Luminescence, Endoscopy, Sensors, Microscopy, Chemical elements, Spatial resolution

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SPIE Journal Paper | 6 February 2012 Open Access

Investigating photoexcitation-induced mitochondrial damage by chemotherapeutic corroles using multimode optical imaging

Jae-Youn Hwang, Daniel Farkas, David Lubow, Jessica Sims, Lali Medina-Kauwe, Harry Gray, Atif Mahammed, Zeev Gross

JBO, Vol. 17, Issue 1, 015003, (February 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.1.015003

KEYWORDS: Luminescence, Tumors, Digital image correlation, Optical imaging, Picosecond phenomena, Fluorescence lifetime imaging, Toxicity, Cancer, Imaging spectroscopy, Absorption

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SPIE Journal Paper | 1 June 2011 Open Access

Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo

Jae-Youn Hwang, Daniel Farkas, Lali Medina-Kauwe, Zeev Gross, Harry Gray

JBO, Vol. 16, Issue 06, 066007, (June 2011) https://doi.org/10.1117/12.10.1117/1.3589299

KEYWORDS: Tumors, Imaging spectroscopy, Luminescence, In vivo imaging, Image classification, Molecules, Breast, Tissues, Skin, Gallium

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Proceedings Article | 28 February 2011 Paper

Polarization-sensitive digital dermoscopy for image processing-assisted evaluation of atypical nevi: towards step-wise detection of melanoma

Lauren Yu, Anika O. N. Joseph, Erik Lindsley, Daniel Farkas

Proceedings Volume 7902, 79020K (2011) https://doi.org/10.1117/12.891083

KEYWORDS: Melanoma, Image processing, Image analysis, Digital image processing, Fractal analysis, Detection and tracking algorithms, Skin, Algorithm development, Hyperspectral imaging, Medical imaging

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Proceedings Article | 28 February 2011 Paper

Multimode optical imaging for translational chemotherapy: in vivo tumor detection and delineation by targeted gallium corroles

Jae Youn Hwang, Zeev Gross, Harry Gray, Lali Medina-Kauwe, Daniel Farkas

Proceedings Volume 7902, 79020F (2011) https://doi.org/10.1117/12.877780

KEYWORDS: Tumors, Luminescence, Optical imaging, In vivo imaging, Imaging spectroscopy, Imaging systems, Tissues, Target detection, Fluorescence lifetime imaging, Gallium

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Proceedings Article | 18 February 2011 Paper

Investigating the photosensitizer-potential of targeted gallium corrole using multimode optical imaging

Jae Youn Hwang, Jay Lubow, David Chu, Zeev Gross, Harry Gray, Daniel Farkas, Lali Medina-Kauwe

Proceedings Volume 7886, 78860M (2011) https://doi.org/10.1117/12.873337

KEYWORDS: Luminescence, Optical imaging, Tumors, Confocal microscopy, Fluorescence lifetime imaging, Breast cancer, Imaging spectroscopy, Photosensitizer targeting, Proteins, Photodynamic therapy

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SPIE Journal Paper | 1 September 2010 Open Access

Biomedical Optical Imaging

Barry Masters, James Fujimoto, Daniel Farkas

JBO, Vol. 15, Issue 05, 059902, (September 2010) https://doi.org/10.1117/12.10.1117/1.3490919

KEYWORDS: Biomedical optics, Optical imaging, Medical research, Optical coherence tomography, Confocal microscopy, Ophthalmology, Tissue optics, Optical microscopy, Imaging spectroscopy, Microscopy

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Proceedings Article | 9 March 2010 Paper

Automatic recognition of abnormal cells in cytological tests using multispectral imaging

A. Gertych, G. Galliano, S. Bose, D. Farkas

Proceedings Volume 7624, 762435 (2010) https://doi.org/10.1117/12.844482

KEYWORDS: Multispectral imaging, Image segmentation, Image classification, Library classification systems, Cervical cancer, Image processing, Feature extraction, Imaging systems, Binary data, Pathology

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Proceedings Article | 25 February 2010 Paper

Real-time megapixel multispectral bioimaging

Jason Eichenholz, Nick Barnett, Yishung Juang, Dave Fish, Steve Spano, Erik Lindsley, Daniel Farkas

Proceedings Volume 7568, 75681L (2010) https://doi.org/10.1117/12.842563

KEYWORDS: Optical filters, Image filtering, Imaging systems, Image processing, Cameras, Radio frequency weapons, Imaging spectroscopy, Charge-coupled devices, Multispectral imaging, Digital filtering

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SPIE Journal Paper | 1 November 2008 Open Access

Quantitative and morphometric evaluation of the angiogenic effects of leptin

Dodanim Talavera-Adame, Yizhi Xiong, Tong Zhao, Ana Arias, M. Rocio Sierra-Honigmann, Daniel Farkas

JBO, Vol. 13, Issue 06, 064017, (November 2008) https://doi.org/10.1117/12.10.1117/1.3028010

KEYWORDS: Content addressable memory, Image analysis, Control systems, Blood vessels, Molecules, In vivo imaging, Surgery, MATLAB, Fractal analysis, Image processing

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Proceedings Article | 18 March 2008 Paper

Large field of view scanning fluorescence lifetime imaging system for multimode optical imaging of small animals

Jae Youn Hwang, Hasmik Agadjanian, Lali Medina-Kauwe, Zeev Gross, Harry Gray, Karn Sorasaenee, Daniel Farkas

Proceedings Volume 6859, 68590G (2008) https://doi.org/10.1117/12.769305

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Imaging systems, Imaging spectroscopy, Optical imaging, Pulsed laser operation, Molecules, Tissues, Preclinical imaging, Optical scanning systems

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Proceedings Article | 28 February 2008 Paper

Characterization of tumor cells and stem cells by differential nuclear methylation imaging

Jian Tajbakhsh, Kolja Wawrowsky, Arkadiusz Gertych, Ori Bar-Nur, Eugene Vishnevsky, Erik Lindsley, Daniel Farkas

Proceedings Volume 6859, 68590F (2008) https://doi.org/10.1117/12.769289

KEYWORDS: Tumors, Stem cells, Cancer, Image analysis, 3D image processing, Confocal microscopy, Visualization, Image filtering, Digital filtering, Surgery

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Proceedings Article | 25 February 2008 Paper

Intelligent noncontact surgeon-computer interface using hand gesture recognition

Michael Zhao, Andreas Nowatzyk, Thomas Lu, Daniel Farkas

Proceedings Volume 6805, 68050U (2008) https://doi.org/10.1117/12.766870

KEYWORDS: Neural networks, Cameras, Surgery, Gesture recognition, Feature extraction, Human-machine interfaces, Light sources and illumination, 3D image processing, Algorithm development, Evolutionary algorithms

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Proceedings Article | 21 February 2008 Paper

Quantitative endoscopic imaging elastic scattering spectroscopy: model system/tissue phantom validation

E. Lindsley, D. Farkas

Proceedings Volume 6870, 68700I (2008) https://doi.org/10.1117/12.769234

KEYWORDS: Tissues, Imaging spectroscopy, Scattering, Optical spheres, Tissue optics, Mie scattering, Data modeling, Endoscopy, Cancer, Titanium dioxide

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Proceedings Article | 19 February 2007 Paper

Contrast enhancement in biomedical optical imaging using ultrabright color LEDs

Kevin Burton, Raphael Zelikowsky, Danny Shandling, Erik Lindsley, Daniel Farkas

Proceedings Volume 6441, 64411I (2007) https://doi.org/10.1117/12.712401

KEYWORDS: Light emitting diodes, Tissues, Lamps, Spatial frequencies, Endoscopy, LED lighting, Microscopes, Microscopy, Endoscopes, Tungsten

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Proceedings Article | 19 February 2007 Paper

Intelligent spectral signature bio-imaging in vivo for surgical applications

Jihoon Jeong, Philip Frykman, Mark Gaon, Alice Chung, Erik Lindsley, Jae Y. Hwang, Daniel Farkas

Proceedings Volume 6441, 64411N (2007) https://doi.org/10.1117/12.712412

KEYWORDS: Colon, Surgery, Databases, Visualization, Tissues, In vivo imaging, Neck, Imaging spectroscopy, Image processing, Animal model studies

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Proceedings Article | 19 February 2007 Paper

Multimode optical imaging of small animals: development and applications

J. Y. Hwang, C. Moffatt-Blue, O. Equils, M. Fujita, J. Jeong, N. Khazenzon, E. Lindsley, J. Ljubimova, A. Nowatzyk, D. Farkas, S. Wachsmann-Hogiu

Proceedings Volume 6441, 644105 (2007) https://doi.org/10.1117/12.711582

KEYWORDS: Imaging systems, Luminescence, Tumors, Tissues, Bioluminescence, Fluorescence lifetime imaging, Optical imaging, Hyperspectral imaging, Breast, Charge-coupled devices

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Proceedings Article | 19 February 2007 Paper

Wide-field two-photon microscopy: features and advantages for biomedical applications

S. Wachsmann-Hogiu, J. Hwang, E. Lindsley, D. Farkas

Proceedings Volume 6441, 64411B (2007) https://doi.org/10.1117/12.711603

KEYWORDS: Luminescence, Confocal microscopy, Objectives, Tissues, Fluorescence lifetime imaging, Picosecond phenomena, Biomedical optics, Microscopy, Blood, Two photon excitation microscopy

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Proceedings Article | 22 February 2006 Paper

Spectral imaging detects breast cancer in fresh unstained specimens

Alice Chung, Mark Gaon, Jihoon Jeong, Scott Karlan, Erik Lindsley, Sebastian Wachsmann-Hogiu, Yizhi Xiong, Tong Zhao, Daniel Farkas

Proceedings Volume 6088, 608806 (2006) https://doi.org/10.1117/12.644927

KEYWORDS: Tissues, Breast cancer, Imaging spectroscopy, Image segmentation, Breast, Cancer, Tumors, Image classification, In vivo imaging, Library classification systems

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Proceedings Article | 21 February 2006 Paper

Acousto-optic tuneable filters: advances and applications to microscopy

C. Pannell, E. Wachman, D. Farkas, J. Ward, W. Seale

Proceedings Volume 6088, 60880Y (2006) https://doi.org/10.1117/12.649587

KEYWORDS: Acoustics, Acousto-optics, Transducers, Optical filters, Diffraction, Crystals, Tunable filters, Scattering, Image quality, Image filtering

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Proceedings Article | 29 April 2005 Paper

A novel node-structural map for angiogenesis analysis

Yizhi Xiong, Tong Zhao, Dodanim Talavera, Rocio Honigmann, Daniel Farkas

Proceedings Volume 5747, (2005) https://doi.org/10.1117/12.596443

KEYWORDS: Content addressable memory, Image segmentation, Blood vessels, In vivo imaging, Quantitative analysis, Image analysis, Control systems, In vitro testing, Arteries, Fractal analysis

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Proceedings Article | 29 March 2005 Paper

Multiphoton, confocal, and lifetime microscopy for molecular imaging in cartilage

Sebastian Wachsmann-Hogiu, Deborah Krakow, Veneta Kirilova, Daniel Cohn, Cristina Bertolotto, Dora Acuna, Qiyin Fang, Nikola Krivorov, Daniel Farkas

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.597670

KEYWORDS: Confocal microscopy, Luminescence, Fluorescence resonance energy transfer, Cartilage, Proteins, Fluorescence lifetime imaging, Multiphoton microscopy, Tissues, Microscopy, Molecular imaging

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Proceedings Article | 29 March 2005 Paper

Improved 3D cellular imaging by multispectral focus assessment

Tong Zhao, Yizhi Xiong, Alice Chung, Elliot Wachman, Daniel Farkas

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.597586

KEYWORDS: Point spread functions, Multispectral imaging, Microscopes, Image segmentation, 3D image processing, Solids, Databases, Image processing, Stereoscopy, Image analysis

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Proceedings Article | 1 July 2004 Paper

Confocal and two-photon imaging in cartilage: expression patterns of Filamin A and B

Sebastian Wachsmann-Hogiu, Deborah Krakow, Eiman Sebald, Cristina Bertolotto, Dora Acuna, Daniel Farkas

Proceedings Volume 5322, (2004) https://doi.org/10.1117/12.561883

KEYWORDS: Confocal microscopy, Cartilage, Tissues, Luminescence, Proteins, Two photon imaging, Laser scanners, Objectives, Microscopy, Digital image correlation

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Proceedings Article | 1 July 2004 Paper

A novel scheme for abnormal cell detection in Pap smear images

Tong Zhao, Elliot Wachman, Daniel Farkas

Proceedings Volume 5318, (2004) https://doi.org/10.1117/12.529770

KEYWORDS: Wavelets, Feature extraction, Image classification, Neural networks, Cervical cancer, Cancer, Distance measurement, Statistical analysis, Imaging systems, Multispectral imaging

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Proceedings Article | 1 July 2004 Paper

A recursive spectral selection scheme for unsupervised segmentation of multispectral Pap smear image sets

Tong Zhao, Elliot Wachman, Stanley Geyer, Daniel Farkas

Proceedings Volume 5322, (2004) https://doi.org/10.1117/12.529775

KEYWORDS: Image segmentation, Multispectral imaging, Distance measurement, Feature selection, Cervical cancer, Machine learning, Computer aided diagnosis and therapy, Tissues, Principal component analysis, Data modeling

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Proceedings Article | 1 July 2004 Paper

The hyperspectral imaging endoscope: a new tool for in vivo cancer detection

Erik Lindsley, Elliot Wachman, Daniel Farkas

Proceedings Volume 5322, (2004) https://doi.org/10.1117/12.561884

KEYWORDS: Endoscopes, Cancer, Cameras, Tissues, Tissue optics, Mie scattering, Biopsy, Scattering, In vivo imaging, Imaging systems

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Proceedings Article | 28 May 2002 Paper

Thermal energy effects on articular cartilage: a multidisciplinary evaluation

Lee Kaplan, John Ernsthausen, Dan Ionescu, Rebecca Studer, James Bradley, Constance Chu, Freddie Fu, Daniel Farkas

Proceedings Volume 4622, (2002) https://doi.org/10.1117/12.468358

KEYWORDS: Cartilage, Thermal effects, Tissues, Temperature metrology, Surgery, Manufacturing, Control systems, Microscopes, Optical imaging, Thermal modeling

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SPIE Journal Paper | 1 January 2001 Open Access

Rhod-2 based measurements of intracellular calcium in the perfused mouse heart: cellular and subcellular localization and response to positive intropy

Guy MacGowan, Congwu Du, Vazha Glonty, Joseph Suhan, Daniel Farkas, Alan Koretsky

JBO, Vol. 6, Issue 01, (January 2001) https://doi.org/10.1117/12.10.1117/1.1316091

KEYWORDS: Calcium, Luminescence, Heart, Absorbance, Confocal microscopy, Microscopy, Tissues, Photomicroscopy, Electron microscopy, Motion measurement

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Proceedings Article | 11 October 2000 Paper

Noninvasive imaging of biological tissue structure, function, and abnormalities with optical coherence tomography

Yingtian Pan, John Lavelle, S. Bastaky, Daniel Farkas, Mark Zeidel

Proceedings Volume 4224, (2000) https://doi.org/10.1117/12.403947

KEYWORDS: Optical coherence tomography, Bladder, Tissues, Tissue optics, Tumors, Scattering, Image resolution, Mirrors, Biopsy, Imaging systems

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Proceedings Article | 3 May 1999 Paper

Quantitative measurement of calcium transients in perfused mouse heart using Rhod-2 fluorometry

Congwu Du, Guy MacGowan, Daniel Farkas, Alan Koretsky

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347510

KEYWORDS: Luminescence, Heart, Calcium, Absorbance, Tissues, Tissue optics, Scattering, Calibration, Absorption, Signal detection

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Proceedings Article | 16 October 1998 Paper

Spectral imaging in biomedicine: a selective overview

Richard Levenson, Elliot Wachman, Wenhua Niu, Daniel Farkas

Proceedings Volume 3438, (1998) https://doi.org/10.1117/12.328111

KEYWORDS: Imaging spectroscopy, Tissues, Spectroscopy, Optical filters, Pathology, Image filtering, Luminescence, Infrared imaging, Infrared radiation, Tunable filters

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SPIE Journal Paper | 1 October 1998 Open Access

Non-invasive Imaging of Living Human Skin with Dual-wavelength Optical Coherence Tomography in Two and Three Dimensions

Yingtian Pan, Daniel Farkas

JBO, Vol. 3, Issue 04, (October 1998) https://doi.org/10.1117/12.10.1117/1.429897

KEYWORDS: Optical coherence tomography, Tissues, Skin, 3D image processing, Scattering, Light scattering, In vivo imaging, Blood vessels, Image resolution, Scanners

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Proceedings Article | 29 April 1998 Paper

Noninvasive imaging of biological tissue with dual-wavelength optical coherence tomography

Yingtian Pan, Daniel Farkas

Proceedings Volume 3260, (1998) https://doi.org/10.1117/12.307109

KEYWORDS: Optical coherence tomography, Tissue optics, Tissues, Speckle, Scanners, Fiber optics, Scattering, Biomedical optics, Tomography, Light sources

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Proceedings Article | 23 May 1997 Paper

Digital spectral imaging for histopathology and cytopathology

Richard Levenson, Daniel Farkas

Proceedings Volume 2983, (1997) https://doi.org/10.1117/12.274331

KEYWORDS: Imaging spectroscopy, Diagnostics, Image segmentation, Tumors, Pathology, Cancer, Tissues, Statistical analysis, Breast cancer, Biopsy

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Proceedings Article | 23 May 1997 Paper

In-vivo imaging of biological tissues using 1.3 um optical coherence tomography

Yingtian Pan, Daniel Farkas

Proceedings Volume 2983, (1997) https://doi.org/10.1117/12.274330

KEYWORDS: Tissues, Skin, Optical coherence tomography, Coherence imaging, In vivo imaging, Scattering, Mirrors, Tissue optics, Diagnostics, Image resolution

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Proceedings Article | 10 May 1996 Paper

Automated interactive microscopy: measuring and manipulating the chemical and molecular dynamics of cells and tissues

D. Lansing Taylor, Lowell Harris, R. DeBiasio, Scott Fahlman, Daniel Farkas, Frederick Lanni, Michel Nederlof, Albert Gough

Proceedings Volume 2678, (1996) https://doi.org/10.1117/12.239522

KEYWORDS: Microscopes, Microscopy, Luminescence, Cameras, Image processing, Imaging systems, Human-machine interfaces, Data acquisition, Image resolution, 3D image processing

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Proceedings Article | 10 May 1996 Paper

Microscopic and mesoscopic spectral bioimaging

Daniel Farkas, Byron Ballou, Gregory Fisher, Daniel Fishman, Yuval Garini, Wenhua Niu, Elliot Wachman

Proceedings Volume 2678, (1996) https://doi.org/10.1117/12.239508

KEYWORDS: Microscopy, Luminescence, Imaging spectroscopy, Tumors, In vivo imaging, Skin, Tissues, Acousto-optics, Tissue optics, Image resolution

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Proceedings Article | 1 April 1996 Paper

Three-dimensional imaging of nucleolin trafficking in normal cells, transfectants, and heterokaryons

Byron Ballou, Gregory Fisher, Jau-Shyong Deng, Thomas Hakala, Meera Srivastava, Daniel Farkas

Proceedings Volume 2680, (1996) https://doi.org/10.1117/12.237600

KEYWORDS: Proteins, Tumors, In vivo imaging, 3D acquisition, Monoclonal antibodies, Tissues, 3D image processing, Microscopes, Systems modeling, Biotechnology

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The study of intracellular trafficking using labeled molecules has been aided by the development of the cyanine fluorochromes, which are easily coupled, very soluble, resist photobleaching, and fluoresce at far-red wavelengths where background fluorescence is minimal. We have used Cy3-, Cy5-, and Cy5.5-labeled antibodies, antigen-binding fragments, and specifically binding single-stranded oligonucleotides to follow expression and trafficking of nucleolin, the most abundant protein of the nucleolus. Nucleolin shuttles between the nucleolus and the cytoplasm, and is also expressed on the cell surface, allowing us to test our techniques at all three cellular sites. Differentially cyanine-labeled non-specific antibodies were used to control for non-specific binding. Similarly, the differentially labeled non-binding strand of the cloned oligonucleotide served as a control. The multimode microscope allowed us to follow both rapid and slow redistributions of labeled ligands in the same study. We also performed 3-D reconstructions of nucleolin distribution in cells using rapid acquisition and deconvolution. Microinjection of labeled ligands was used to follow intracellular distribution, while incubation of whole cells with antibody and antigen-binding fragments was used to study uptake. To unambiguously define trafficking, and eliminate the possibility of interference by cross-reactive proteins, we transfected mouse renal cell carcinoma cells that express cell surface nucleolin with human nucleolin. We used microinjection and cell surface staining with Cy3- or Cy5- labeled monoclonal antibody D3 (specific for human nucleolin) to assess the cellular distribution of the human protein. Several clones expressed human nucleolin on their surfaces and showed high levels of transport of the human protein into the mouse nucleus and nucleolus. This distribution roughly parallels that of mouse nucleolin as determined by labeled polyclonal antibody. We have used these engineered transfectants to determine whether the cell surface-expressed xenogeneic nucleolin can serve as a target for antibodies in vivo.

Proceedings Article | 3 April 1995 Paper

From in vitro to in vivo by dynamic multiwavelength imaging

Daniel Farkas, Byron Ballou, Gregory Fisher, D. Lansing Taylor

Proceedings Volume 2386, (1995) https://doi.org/10.1117/12.206015

KEYWORDS: Tumors, Microscopy, In vivo imaging, In vitro testing, Luminescence, Tissues, Microscopes, Monoclonal antibodies, Live cell imaging, Imaging systems

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Proceedings Article | 17 August 1994 Paper

New waves in light microscopy

Daniel Farkas, Brent Bailey, Frederick Lanni, D. Lansing Taylor

Proceedings Volume 2137, (1994) https://doi.org/10.1117/12.182704

KEYWORDS: Microscopy, Microscopes, Luminescence, 3D image processing, Image processing, Confocal microscopy, Sensors, Image resolution, Imaging systems, 3D acquisition

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Proceedings Article | 4 April 1994 Paper

Three-dimensional imaging of biological specimens with standing wave fluorescence microscopy

Brent Bailey, Vijay Krishnamurthi, Daniel Farkas, D. Lansing Taylor, Frederick Lanni

Proceedings Volume 2184, (1994) https://doi.org/10.1117/12.172101

KEYWORDS: Microscopy, Luminescence, 3D image processing, Microscopes, Refraction, Image resolution, Yeast, Modulation, Mirrors, Image processing

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Showing 5 of 59 publications

Proceedings Volume Editor (26)

SPIE Conference Volume | 4 March 2020

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII

SPIE Conference Volume | 7 June 2019

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII

SPIE Conference Volume | 2 May 2018

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI

SPIE Conference Volume | 21 April 2017

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV

SPIE Conference Volume | 17 June 2016

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX

Showing 5 of 26 publications

Conference Committee Involvement (34)

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII

3 February 2020 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII

4 February 2019 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI

29 January 2018 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV

30 January 2017 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX

15 February 2016 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII

9 February 2015 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII

3 February 2014 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI

2 February 2013 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X

21 January 2012 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX

22 January 2011 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII

23 January 2010 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII

26 January 2009 | San Jose, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI

21 January 2008 | San Jose, California, United States

Advanced Biomedical and Clinical Diagnostic Systems VI

20 January 2008 | San Jose, California, United States

Confocal, Multiphoton, and Nonlinear Microscopic Imaging

17 June 2007 | Munich, Germany

Imaging, Manipulation and Analysis of Biomolecules, Cells, and Tissues V

22 January 2007 | San Jose, California, United States

Advanced Biomedical and Clinical Diagnostic Systems V

21 January 2007 | San Jose, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV

23 January 2006 | San Jose, California, United States

Advanced Biomedical and Clinical Diagnostic Systems IV

22 January 2006 | San Jose, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II

24 January 2005 | San Jose, CA, United States

Advanced Biomedical and Clinical Diagnostic Systems III

23 January 2005 | San Jose, CA, United States

Imaging, Manipulation and Analysis of Biomolecules, Cells, and Tissues

27 January 2004 | San Jose, CA, United States

BioMEMS and Nanotechnology

10 December 2003 | Perth, Australia

Confocal, Multiphoton, and Nonlinear Microscopic Imaging

22 June 2003 | Munich, Germany

Manipulation and Analysis of Biomolecules, Cells and Tissues

28 January 2003 | San Jose, CA, United States

Advanced Biomedical and Clinical Diagnostic Systems

26 January 2003 | San Jose, CA, United States

Optical Diagnostics of Living Cells V

23 January 2002 | San Jose, CA, United States

Optical Diagnostics of Living Cells IV

24 January 2001 | San Jose, CA, United States

Functional Imaging and Optical Manipulation of Living Cells and Tissues

24 January 2000 | San Jose, CA, United States

Optical Diagnostics of Living Cells III

24 January 2000 | San Jose, CA, United States

Functional Imaging and Optical Manipulation of Living Cells

25 January 1999 | San Jose, CA, United States

Optical Diagnostics of Living Cells II

25 January 1998 | San Jose, CA, United States

Functional Imaging and Optical Manipulation of Living Cells

10 February 1997 | San Jose, CA, United States

Optical Diagnostics of Living Cells and Biofluids

31 January 1996 | San Jose, CA, United States

Showing 5 of 34 Conference Committees

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