Prof. Dar-Bin Shieh Profile

Prof. Dar-Bin Shieh

at National Cheng Kung Univ

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Author

Publications (4)

Proceedings Article | 21 February 2020 Presentation + Paper

Sensing of diseased mitochondria proportion by DEP at the organelle level of intact cells

Pei-Yin Chi, Ting-Wei Chuang, Tzu-Tsai Chu, Chia-Tzu Kuo, Yu-Ting Wu, Vahid Farmehini, Dar-Bin Shieh, Fan-Gang Tseng, Yau-Huei Wei, Nathan Swami, Chia-Fu Chou

Proceedings Volume 11235, 112350N (2020) https://doi.org/10.1117/12.2540653

KEYWORDS: Dielectrophoresis, Diamond, Silicon, Microfluidics, Medicine, Proteins, Dielectrics, Plasma, Algorithm development, Sensing systems

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Proceedings Article | 26 February 2010 Paper

In vivo optical virtual biopsy of human oral cavity with harmonic generation microscopy

M.-R. Tsai, S.-Y. Chen, D.-B. Shieh, P.-J. Lou, C.-K. Sun

Proceedings Volume 7569, 75691Q (2010) https://doi.org/10.1117/12.842223

KEYWORDS: Tissue optics, In vivo imaging, Harmonic generation, Biopsy, Second-harmonic generation, Microscopy, Cancer, Collagen, Capillaries, Image enhancement

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Proceedings Article | 11 March 2008 Paper

In vivo photoacoustic imaging with multiple selective targeting using bioconjugated gold nanorods

Chen-Wei Wei, Chao-Kang Liao, Ying-Yi Chen, Churng-Ren Chris Wang, Ann-Ann Ding, Dar-Bin Shiehd, Pai-Chi Li

Proceedings Volume 6856, 68560J (2008) https://doi.org/10.1117/12.764454

KEYWORDS: Gold, Tumors, Cancer, Nanoprobes, In vivo imaging, Absorption, Photoacoustic imaging, Target detection, Photoacoustic spectroscopy, Nanorods

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Proceedings Article | 6 March 2006 Paper

Multiple targeting in photoacoustic imaging using bioconjugated gold nanorods

Pai-Chi Li, Chen-Wei Wei, Chao-Kang Liao, Cheng-Dah Chen, Kuei-Chen Pao, Churng-Ren Chris Wang, Ya-Na Wu, Dar-Bin Shieh

Proceedings Volume 6086, 60860M (2006) https://doi.org/10.1117/12.645248

KEYWORDS: Gold, Absorption, Nanoparticles, Proteins, Cancer, Photoacoustic spectroscopy, Molecules, Nanoprobes, Photoacoustic imaging, Nanorods

Read Abstract +

Cancer cells presented altered surface molecules to encourage their growth and metastasis. Expression of oncogeneic surface molecules also play important roles in the prediction of clinical outcome and treatment response of anti-cancer drugs. It is thus conceivable that imaging of cancer lesions while simultaneously obtaining their pathogenic information at molecular level of as many oncogenic proteins as possible is of great clinical significance. Gold nanoparticles have been used as a contrast agent for photoacoustic imaging. In addition, gold nanoparticles can be bioconjugated to probe certain molecular processes. An intriguing property of gold nanoparticles is its ability to tailor its optical properties. For example, size effects on the surface plasmon absorption of spherical gold nanoparticles have shown that the peak optical absorption red-shifts with the increasing particle size. In addition, the optical absorption spectrum of cylindrical gold nanoparticles (i.e., gold nanorods) exhibits a strong absorption band that is directly related to the aspect ratio. With these unique characteristics, selective targeting can be achieved in photoacoustic molecular imaging. Specifically, gold nanorods with different aspect ratios can be bioconjugated to different antibodies. Multiple targeting and simultaneous detection can then be achieved by using laser irradiation at the respective peak optical absorption wavelength. In this study, photoacoustic multiple targeting using gold nanorods is experimentally demonstrated. We have chosen Her2 and CXCR4 as our primary target molecule as Her2 expression is associated with growth characteristics and sensitivity to Herceptin chemotherapy. On the other hand, CXCR4 expression predict the organ-specific metastatic potential of the cancer cells for clinical intervention in advance. Monoclonal antibody (mAb) against Her2/neu was conjugated to nanorods with several different aspect ratios. The agarose gel is suitable for photoacoustic signal acquisition. A wavelength tunable Ti-Sapphire laser was used for laser irradiation and a 1 MHz ultrasound transducer was used for acoustic detection. The optical wavelength of the laser was tuned between 800 nm and 940 nm, corresponding to gold nanorods of an aspect ratio ranging from 3.7 to 5.9. The results clearly show the potential of photoacoustic molecular imaging with multiple targeting in revealing different oncogene expression levels of the cancer cells.

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