Increased cellular metabolic activity can be quantified by imaging the oral tissue autofluorescence originated from NADH and FAD. Clinical label-free metabolic imaging of oral epithelial cancer based on 375-nm excitation maFLIM endoscopy was recently demonstrated in patients presenting oral malignant lesions. A recently upgraded maFLIM endoscopy system enables simultaneous autofluorescence excitation at 375 nm (for NADH) and 445 nm (for FAD). This maFLIM endoscope is being used to clinically image healthy, benign, precancerous and cancerous oral lesions in oral health care settings. Comprehensive statistical analysis will be performed to identify new metabolic and biochemical autofluorescence biomarkers of oral epithelial cancer.
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