This study demonstrates the possibility of using the SERS method to detect methotrexate (MTX) molecules in the blood plasma of patients in concentrations up to 10-6 M. The paper performs investigation for samples from patients who took the drug at different doses. Borosilicate glasses coated with silver nanoparticles were used as the surface. We analyzed the differences between the spectra of patients after taking medication and healthy volunteers without taking medication. The characteristic maxima of methotrexate were determined.
This study demonstrates the possibility of the surface enhanced Raman spectroscopy (SERS) method to determine concentrations of methotrexate (MTX) in human plasma. This method makes it possible to detect low concentrations of methotrexate in biological samples with relatively inexpensive portable equipment. The SERS signal may be greatly enhanced using nanostructured plasmonic materials which will make this method highly sensitive, selective and productive. The use of SERS to perform a therapeutic drug monitoring procedure is a promising method because of its extreme sensitivity, specificity and speed of analysis.
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