A proposed transcutaneous monitoring system utilizing a CO2 sensor with ion-selective electrodes for monitoring transcutaneous carbon dioxide partial pressure (TcpCO2) is detailed in this study. The system incorporates an STM32F103 processor as the control chip, designed with a modular solution. The hardware circuit design scheme include power supply circuit, PID temperature control circuit, signal conditioning circuit, digital-analog conversion. Additionally, an Microsoft Foundation Classes(MFC)-based human-computer interface is designed for data communication, display and storage. The above system was validated through experimentation, demonstrating that the designed TcpCO2 monitoring system can achieve a temperature measurement accuracy of ±0.1o C and a temperature control accuracy of ±0.2o C. Furthermore, the system was able to detect changes in partial pressure of CO2 (pCO2) within the range of 0-100 mmHg, with a detection limit(LOD) of 1.38 mmHg and a relative standard deviation(RSD) of 7.07% for pCO2. Furthermore, the electrochemical workstation system, initially small and simple, has been enhanced with the addition of a weak current detection channel and a constant potentiostat circuit, thereby enabling the system to broaden its functionality. Ultimately, this system shows promising potential for application in clinical medicine and health monitoring
Observation of actin in cells is crucial for the investigation of cell motility, protein transcription, pathogen infection, and cell cycle research. In this study, we have identified Brilliant Green (BG) as a water-soluble AIEgen (luminogen with aggregation-induced emission properties) that can be utilized for actin staining. Our experimental findings highlight the effectiveness of BG for long-term live cell imaging, as well as its remarkable stability and resistance to photobleaching.
This paper proposes an improved digital microfluidics (DMF) chip based on a printed circuit board (PCB) to realize the precise control of droplets. Solder mask layer was introduced to the chip as the dielectric layer, and this way can simplify the preparation processes of the chip, and the chip offers reasonable control of the droplet. Combined with the external module, the S protein peptide was screened on the chip. As a result, this PCB-based chip provided a new tool for droplet manipulation. It may have potential applications in drug screening, especially in the field with many repetitive operations.
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