We have developed a new µOCT tethered Capsule Endomicroscopy imaging system for the diagnosis of Inflammatory bowel disease and Gastrointestinal cancer with micrometer resolution. The capsule we built measures 30 mm in length and 11 mm in diameter. By employing an optical design that incorporates the mirror tunnel design, we achieved a lateral resolution of less than 4 µm across a depth of field of 450 µm. The spectrometer we designed provides a resolution of 1.8 µm over a 4 mm imaging range, with an A-scan rate of up to 300 kHz. Imaging performance is demonstrated by beam profiling and imaging esophagus ex vivo.
We present the compact SECM system with a 3D-printed tethered endoscopic capsule with a 1060 nm center wavelength. Compared to the previously presented 1310 nm SECM system, the present SECM system theoretically provides 15% improved lateral resolution, 5.2 times faster imaging speed, and two times faster fabrication times. We performed in vivo imaging on the swine esophagus and the SECM images clearly visualized squamous cell nuclei as a bright dot surrounded by dark cytoplasm and bright cell borders. These results indicate that our new SECM endoscopic system making it easier to conduct cellular-level diagnosis of upper GI tract.
We introduce two multimodal extensions of our optical coherence tomography (OCT) based tethered capsule endomicroscopy (TCE) platform, tailored towards an enhanced clinical applicability for upper gastrointestinal tract imaging. The first extension presented – white light (RGB)-OCT-based TCE – provides both, true-color visualization of the tissue surface and depth-resolved sub-surface OCT imaging, co-registered in time and space. The second extension presented – fluorescence (FL)-OCT-based TCE – enables the addition of tissue specific molecular contrast. We present compact, modular, easily portable, plug-and-play system designs for both extensions, as well as imaging results in swine esophagus, in vivo.
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