Dr. Ekaterina N. Lazareva Profile

Dr. Ekaterina N. Lazareva

Lead Engineer at Saratov State Univ

SPIE Involvement:

Author

Publications (19)

Proceedings Article | 27 November 2023 Paper

Tissue optical clearing for fNIRS studies in radiotherapy

Ekaterina Lazareva, Priya Karthikeyan, Jesse Lohela, Kalle Inget, Vesa Korhonen, Sakari Karhula, Juha Nikkinen, Valery Tuchin, Teemu Myllyla

Proceedings Volume 12770, 1277014 (2023) https://doi.org/10.1117/12.2687657

KEYWORDS: Radiotherapy, Tissues, Optical clearing, Absorbance, Brain, X-rays, Skin, Light sources, Brain tissue, Spectroscopy

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Proceedings Article | 19 May 2022 Presentation + Paper

Influence of annealing temperature on the cytotoxicity upconversion nanoparticles on different cell lines

R. Verkhovskii, R. Anisimov, M. Lomova, D. Tuchina, E. Lazareva, A. Doronkina, A. Mylnikov, N. Navolokin, V. Kochubey, I. Yu. Yanina

Proceedings Volume 12147, 121470H (2022) https://doi.org/10.1117/12.2621543

KEYWORDS: Cancer, Erbium, In vitro testing, Ytterbium, Temperature metrology, Upconversion, Annealing, Nanoparticles, Photodynamic therapy, Particles

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Proceedings Article | 29 April 2022 Paper

Measurement of the refractive index of the gray matter of the cow's brain at wavelengths of 480-1550 nm when exposed to different temperatures.

Alaa Shanshool, Ekaterina Lazareva, Valery Tuchin

Proceedings Volume 12192, 1219212 (2022) https://doi.org/10.1117/12.2634156

KEYWORDS: Tissues, Brain, Refractive index, Water, Optical properties, Temperature metrology, Scattering, Biological research, Absorption, Diagnostics

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Proceedings Article | 2 March 2022 Poster + Paper

Assessment of cytotoxicity upconversion nanoparticles coated by SiO2 on different cell lines

R. Verkhovskii, R. Anisimov, M. Lomova, D. Tuchina, E. Lazareva, A. Doronkina, A. Mylnikov, N. Navolokin, V. Kochubey, I. Yu. Yanina

Proceedings Volume 11959, 1195908 (2022) https://doi.org/10.1117/12.2616509

KEYWORDS: Nanoparticles, Upconversion, Photodynamic therapy, Particles, Silica, Ytterbium, In vitro testing, Erbium, Cancer, Proteins

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Proceedings Article | 8 April 2020 Presentation

THz properties of rat skin and extracts at exposure to hyperosmotic solutions (Conference Presentation)

Ekaterina Lazareva, Maxim Nazarov, Alla Bucharskaya, Valery Tuchin, Alexander Shkurinov

Proceedings Volume 11363, 1136328 (2020) https://doi.org/10.1117/12.2560376

KEYWORDS: Tissue optics, Skin, Terahertz radiation, Optical clearing, Tumors, Scattering, Absorption, Imaging spectroscopy, Glucose, Water

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Application of many optical methods, such as photoacoustics, optical coherent tomography, and terahertz spectroscopy, for tissue imaging and disease diagnostics is limited due to small probing depth caused by strong scattering and absorption in tissues [1, 2]. It is possible to increase the efficiency of optical techniques by using tissue optical clearing (TOC), which allows one to control tissue optical properties by exposing tissue to biocompatible liquids - optical clearing agents (OCAs). When exposed to an OCA with hyperosmotic properties, such as glycerol or highly concentrated glucose solution, it is possible to achieve a considerable decrease of tissue scattering within the entire spectral range and absorption on the water bands due to refractive index matching phenomenon and dehydration of the tissue, and therefore to get a significant increase of the optical and THz wave transmittance [1-3]. In vitro studies were performed on laboratory rats of the Vistar strain (sexually mature females weighing 300–400 g were used). The animals were inoculated with the tumor cells by subcutaneous injection into the area of the scapulae. Studies were conducted in 28 days after injection. The weight and thickness of the samples were measured before and after optical clearing by using a micrometer with an accuracy of ±0.001 mm and an analytical balance (Scientech, SA210, USA) with an accuracy of ±1 mg. The samples of the area 1.5*1.5 cm2 were placed in a closed sealed container, to which was added 2 ml of OCA. 70% glycerol solution, 40% glucose solution and 50% fructose in a 30% alcohol solution were used with the addition of saline as an OCA. The transmission spectrum of glycerol in the frequency range of 0.05–1.0 THz was measured by using the terahertz (THz) time-domain spectrometer. Measurements of the refractive index of OCA were carried out before the start of the experiment and every 5 minutes after placing the skin sample in the solution with OCA for 15 minutes, then every 10 minutes for another 45 minutes. It was found that the effectiveness of optical clearing of the skin over the tumor is higher compared to healthy skin. A good correlation was shown between the kinetics of the sample mass and THz absorption and refraction. An assessment of skin dehydration also showed that the concentration of water extracted from the skin above the tumor is greater compared to healthy. It is especially important that enhancing the image contrast due to dehydration and TOC in the pathology area is extremely significant in diagnosing precancerous conditions and treating early stages of tumor, due to the possibility of selective increase of the scattering contrast of cell nuclei relative to surrounding cytoplasm and interstitial fluid and thus following their size and internal rearrangements [5].

Proceedings Article | 2 April 2020 Presentation + Paper

Binding of ceruloplasmin with cationic porphyrins: pH and salt composition of a medium

Aram Gyulkhandanyan, Anna Zakoyan, Lusine Mkrtchyan, Anna Gyulkhandanyan, Marina Parkhats, Boris Dzhagarov, Marina Sheyranyan, Gegham Simonyan, Ekaterina Lazareva, Valery Tuchin, Grigor Gyulkhandanyan

Proceedings Volume 11363, 1136329 (2020) https://doi.org/10.1117/12.2556021

KEYWORDS: Proteins, Absorption, Tumors, Luminescence, Blood, Molecules, Oxygen, Photodynamic therapy, Macromolecules

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Proceedings Article | 26 March 2020 Presentation

In vivo optical clearing of human light and dark skin (Conference Presentation)

Valery Tuchin, Elina Genina, Alexey Bashkatov, Ekaterina Lazareva, Vladimir Zharov

Proceedings Volume 11239, 1123902 (2020) https://doi.org/10.1117/12.2566932

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Proceedings Article | 22 July 2019 Paper

Ceruloplasmin: a potential carrier of photosensitizers for photodynamic therapy of tumors

Anna Gyulkhandanyan, Anna Zakoyana, Aram Gyulkhandanyan, Marina Parkhots, Boris Dzhagarov, Ekaterina Lazareva, Valery Tuchin, Grigor Gyulkhandanyana

Proceedings Volume 11079, 110791T (2019) https://doi.org/10.1117/12.2527568

KEYWORDS: Photodynamic therapy, Proteins, Tumors, Molecules, Cancer, Blood, Copper, Quantum efficiency, Absorption, Biochemistry

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Proceedings Article | 3 June 2019 Paper

Research and development of effective optical technologies for diagnostics in dermatology

Vadim Genin, Prisca Rakotomanga, Sergey Zaytsev, Elina Genina, Ekaterina Lazareva, Grégoire Khairallah, Marine Amouroux, Charles Soussen, Hang Chen, Wei Feng, Dan Zhu, Alexey Bashkatov, Walter Blondel, Valery Tuchin

Proceedings Volume 11065, 1106505 (2019) https://doi.org/10.1117/12.2528700

KEYWORDS: Skin, Optical coherence tomography, Tissue optics, Luminescence, In vivo imaging, Scattering, Absorption, Optical clearing, Photons, Transparency

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Proceedings Article | 1 March 2019 Presentation + Paper

Medical diagnosis using NIR and THz tissue imaging and machine learning methods

Yury Kistenev, Valery Tuchin, Alexey Borisov, Ekaterina Lazareva, Viktor Nikolaev, Daria Tuchina, Denis Vrazhnov, Irina Yu Yanina

Proceedings Volume 10877, 108770J (2019) https://doi.org/10.1117/12.2508166

KEYWORDS: Tissue optics, Tissues, Machine learning, Terahertz radiation, Medical diagnostics, Skin, Near infrared, Water, Image processing, Tumors

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SPIE Journal Paper | 23 August 2018 Open Access

Measurement of tissue optical properties in the context of tissue optical clearing

Alexey Bashkatov, Kirill Berezin, Konstantin Dvoretskiy, Maria Chernavina, Elina Genina, Vadim Genin, Vyacheslav Kochubey, Ekaterina Lazareva, Alexander Pravdin, Marina Shvachkina, Polina Timoshina, Daria Tuchina, Dmitry Yakovlev, Irina Yu Yanina, Olga Zhernovaya, Valery Tuchin

JBO, Vol. 23, Issue 09, 091416, (August 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.9.091416

KEYWORDS: Tissue optics, Tissues, Optical properties, Skin, Collagen, Blood, Optical clearing, Optical coherence tomography, Optical testing, Scattering

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Proceedings Article | 17 May 2018 Paper

Refraction, fluorescence, and Raman spectroscopy of normal and glycated hemoglobin

Ekaterina Lazareva, Andrey Zyubin, Ilya Samusev, Vasiliy Slezhkin, Vyacheslav Kochubey, Valery Tuchin

Proceedings Volume 10685, 1068540 (2018) https://doi.org/10.1117/12.2307102

KEYWORDS: Raman spectroscopy, Luminescence, Refractive index, Blood, Proteins, Temperature metrology, Glucose, Optical properties, Refraction, Biomedical optics

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SPIE Journal Paper | 15 March 2018 Open Access

Measurement of refractive index of hemoglobin in the visible/NIR spectral range

Ekaterina Lazareva, Valery Tuchin

JBO, Vol. 23, Issue 03, 035004, (March 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.3.035004

KEYWORDS: Blood, Spectroscopy, Refractive index, Near infrared, Temperature metrology, Refraction, Absorption, Microscopy, Tissue optics, Water

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Proceedings Article | 21 April 2016 Paper

The temperature dependence of refractive index of hemoglobin at the wavelengths 930 and 1100 nm

Ekaterina Lazareva, Valery Tuchin

Proceedings Volume 9917, 99170U (2016) https://doi.org/10.1117/12.2234181

KEYWORDS: Refractive index, Temperature metrology, Water, Near infrared, Biomedical optics, Molecules, Refraction, Laser therapeutics, Blood, Data modeling

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