Extracellular vesicles (EVs) are small, sub-micron membrane-bound particles that function in cell-to-cell communication and have potential to be used in diagnostics, therapy, and biological investigations. However, common characterization methods for EVs lack functional information and rely on “bulk” metrics that lack single-EV resolution. Recent work has applied label-free multimodal nonlinear optical microscopy for characterization of EVs via NAD(P)H and FAD autofluorescence. Here, we characterize EVs isolated from urine and serum from human breast cancer surgery patients and breast reduction surgery patients who have no history of breast cancer to examine altered cancer-related metabolic signatures in cancer-associated EVs.
Extracellular vesicles (EVs) are plasma-membrane formed particles released by cells, and range in diameter from 50 to 2000 nm. Interest in EVs is growing, and recent work has aimed to employ nonlinear optical microscopy techniques to better characterize the size, function, and biochemical makeup of EVs. Previous studies have shown that EVs can modulate gene expression and metabolism of cells that uptake them. Here, we use fluorescence lifetime imaging microscopy (FLIM) of reduced nicotinamide adenine dinucleotide and reduced nicotinamide adenine dinucleotide phosphate (NAD(P)H) to monitor the metabolic response of macrophages and other cells to native and foreign EVs and other known cellular activators.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.