The tetrapyrrole chlorin e6 (Ce6) is actively used in photodynamic therapy (PDT) and as a test drug for sonodynamic therapy due to its ability to generate singlet oxygen. In this work, we have analyzed the absorption, photoluminescence (PL), and PL excitation spectra of Ce6 molecules in a nutrient medium and inside the melanoma cells. An analysis of the results shows that Ce6 inside the cancer cells with a low pH remains in monomeric form. A photodynamic test has also been conducted that confirmed the photodynamic effect produced by Ce6 in cancer cells by changing the signals from Ce6 and cells in the absorption and PL spectra.
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